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Literature summary extracted from

  • Shevchik, V.E.; Condemine, G.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N.
    The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937 (1999), J. Bacteriol., 181, 3912-3919.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.6
-
 Dickeya chrysanthemi
4.2.2.9
-
 Dickeya chrysanthemi

General Stability

EC Number General Stability Organism
4.2.2.9 Ca2+ stablizes Dickeya chrysanthemi
4.2.2.9 Mn2+ stablizes Dickeya chrysanthemi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.6 EDTA
-
 Dickeya chrysanthemi
4.2.2.9 EDTA
-
 Dickeya chrysanthemi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.6 0.12
-
 trigalacturonic acid
-
 Dickeya chrysanthemi
4.2.2.6 0.15
-
 digalacturonic acid
-
 Dickeya chrysanthemi
4.2.2.9 additional information
-
 additional information
-
 Dickeya chrysanthemi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.6 cytoplasm
-
 Dickeya chrysanthemi 5737
-
4.2.2.9 cytoplasm
-
 Dickeya chrysanthemi 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.6 Co2+ strong activation Dickeya chrysanthemi
4.2.2.6 Fe2+ strong activation Dickeya chrysanthemi
4.2.2.6 Mn2+ best cofactor, optimal concentration: 0.1 mM Dickeya chrysanthemi
4.2.2.6 Ni2+ strong activation Dickeya chrysanthemi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.6 44124
-
 x * 44124, calculation from nucleotide sequence Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.6 Dickeya chrysanthemi
-
 3937
-
4.2.2.6 Dickeya chrysanthemi 3937
-
 3937
-
4.2.2.9 Dickeya chrysanthemi
-
 3937
-
4.2.2.9 Dickeya chrysanthemi 3937
-
 3937
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.6 digalacturonic acid
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.6 digalacturonic acid
-
 Dickeya chrysanthemi 3937 ?
-
 ?
4.2.2.6 tetragalacturonic acid
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.6 tetragalacturonic acid
-
 Dickeya chrysanthemi 3937 ?
-
 ?
4.2.2.6 trigalacturonic acid
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.6 trigalacturonic acid
-
 Dickeya chrysanthemi 3937 ?
-
 ?
4.2.2.9 polygalacturonate
-
 Dickeya chrysanthemi unsaturated digalacturonate
-
 ?
4.2.2.9 tetragalacturonic acid
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.9 tetragalacturonic acid
-
 Dickeya chrysanthemi 3937 ?
-
 ?
4.2.2.9 trigalacturonic acid
-
 Dickeya chrysanthemi ?
-
 ?
4.2.2.9 trigalacturonic acid
-
 Dickeya chrysanthemi 3937 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.6 ? x * 44124, calculation from nucleotide sequence Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.9 45
-
 Mn2+ or Co2+ increases half-life from 4 min to 15 min Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.6 7.3 7.7
-
 Dickeya chrysanthemi