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Literature summary extracted from

  • Hiyama, K.; Okada, S.
    Action of chondroitinases. III. Ionic strength effects and kinetics in the action of chondroitinase AC (1977), J. Biochem., 82, 429-436.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.5 Cu2+
-
 Paenarthrobacter aurescens
4.2.2.5 dermatan sulfate
-
 Paenarthrobacter aurescens
4.2.2.5 Fe2+
-
 Paenarthrobacter aurescens
4.2.2.5 Pb2+
-
 Paenarthrobacter aurescens
4.2.2.5 Sn2+
-
 Paenarthrobacter aurescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.5 additional information
-
 additional information effect of temperature in the Km-value Pedobacter heparinus
4.2.2.5 additional information
-
 additional information effect of temperature in the Km-value Paenarthrobacter aurescens
4.2.2.5 0.1
-
 chondroitin 4-sulfate 20°C Pedobacter heparinus
4.2.2.5 0.121
-
 chondroitin 6-sulfate 20°C Pedobacter heparinus
4.2.2.5 0.215
-
 chondroitin 6-sulfate 20°C Paenarthrobacter aurescens
4.2.2.5 0.217
-
 hyaluronate 20°C Pedobacter heparinus
4.2.2.5 0.239
-
 chondroitin 20°C Paenarthrobacter aurescens
4.2.2.5 0.239
-
 hyaluronate 20°C Paenarthrobacter aurescens
4.2.2.5 0.273
-
 chondroitin 20°C Pedobacter heparinus
4.2.2.5 0.376
-
 chondroitin 4-sulfate 20°C Paenarthrobacter aurescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.5 Ba2+ activates more effectively than univalent cations Paenarthrobacter aurescens
4.2.2.5 Ca2+ activates more effectively than univalent cations Paenarthrobacter aurescens
4.2.2.5 Co2+ activates more effectively than univalent cations Paenarthrobacter aurescens
4.2.2.5 K+ activates Paenarthrobacter aurescens
4.2.2.5 Mg2+ activates more effectively than univalent cations Paenarthrobacter aurescens
4.2.2.5 Na+ activates Paenarthrobacter aurescens

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Paenarthrobacter aurescens
-
-
-
4.2.2.5 Pedobacter heparinus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin
-
 Pedobacter heparinus ?
-
 ?
4.2.2.5 chondroitin
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 hyaluronate
-
 Paenarthrobacter aurescens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.5 additional information
-
 additional information effect of temperature in the turnover number Pedobacter heparinus
4.2.2.5 additional information
-
 additional information effect of temperature in the turnover number Paenarthrobacter aurescens
4.2.2.5 308
-
 chondroitin 4-sulfate 20°C Pedobacter heparinus
4.2.2.5 345
-
 chondroitin 6-sulfate 20°C Pedobacter heparinus
4.2.2.5 403
-
 chondroitin 4-sulfate 20°C Paenarthrobacter aurescens
4.2.2.5 522
-
 chondroitin 6-sulfate 20°C Paenarthrobacter aurescens
4.2.2.5 570
-
 hyaluronate 20°C Pedobacter heparinus
4.2.2.5 840
-
 chondroitin 20°C Pedobacter heparinus
4.2.2.5 868
-
 chondroitin 20°C Paenarthrobacter aurescens
4.2.2.5 1200
-
 hyaluronate 20°C Paenarthrobacter aurescens