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Literature summary extracted from
- Purification and properties of bacterial chondroitinases and chondrosulfatases (1968), J. Biol. Chem., 243, 1523-1535.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.10 | acetate | - |
Proteus vulgaris |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.21 | Cu2+ | - |
Pedobacter heparinus | |
| 4.2.2.21 | Fe2+ | - |
Pedobacter heparinus | |
| 4.2.2.21 | heparin | - |
Pedobacter heparinus | |
| 4.2.2.21 | heparitin sulfate | - |
Pedobacter heparinus | |
| 4.2.2.21 | keratosulfate | - |
Pedobacter heparinus | |
| 4.2.2.21 | Zn2+ | - |
Pedobacter heparinus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.21 | Ca2+ | - |
Pedobacter heparinus | |
| 4.2.2.21 | Mg2+ | - |
Pedobacter heparinus | |
| 4.2.2.21 | Mn2+ | - |
Pedobacter heparinus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.21 | 150000 | - |
or slightly lower, gel filtration | Proteus vulgaris |
| 4.2.2.21 | 150000 | - |
or slightly lower, gel filtration | Pedobacter heparinus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | Proteus vulgaris | acts on saturated and unsaturated analogue and N-acetyl-D-galactosamine 4,6-disulfate, but not on higher oligosaccharides nor any 4-sulfate | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | Proteus vulgaris NCTC 4636 | acts on saturated and unsaturated analogue and N-acetyl-D-galactosamine 4,6-disulfate, but not on higher oligosaccharides nor any 4-sulfate | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.10 | Proteus vulgaris | - |
- |
- |
| 4.2.2.21 | Pedobacter heparinus | - |
- |
- |
| 4.2.2.21 | Proteus vulgaris | - |
growth on medium containing chondroitin sulfate C | - |
| 4.2.2.21 | Proteus vulgaris NCTC 4636 | - |
growth on medium containing chondroitin sulfate C | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.10 | copurification with chondroitinase ABC | Proteus vulgaris |
| 3.1.6.10 | partial | Proteus vulgaris |
| 4.2.2.21 | - |
Proteus vulgaris |
| 4.2.2.21 | - |
Pedobacter heparinus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.10 | 4.75 | - |
- |
Proteus vulgaris |
| 4.2.2.21 | 37.5 | - |
pH 8.0, 37°C | Proteus vulgaris |
| 4.2.2.21 | 43 | - |
pH 8.0, 37°C | Pedobacter heparinus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | - |
Proteus vulgaris | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | acts on saturated and unsaturated analogue and N-acetyl-D-galactosamine 4,6-disulfate, but not on higher oligosaccharides nor any 4-sulfate | Proteus vulgaris | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | - |
Proteus vulgaris NCTC 4636 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
| 3.1.6.10 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine 6-sulfate + H2O | acts on saturated and unsaturated analogue and N-acetyl-D-galactosamine 4,6-disulfate, but not on higher oligosaccharides nor any 4-sulfate | Proteus vulgaris NCTC 4636 | 4-deoxy-beta-D-gluc-4-enuronosyl-(1,4)-N-acetyl-D-galactosamine + sulfate + 2 H+ | - |
? | |
| 3.1.6.10 | acetylchondrosin 6-sulfate + H2O | saturated analogue | Proteus vulgaris | acetylchondrosin + sulfate | - |
? | |
| 3.1.6.10 | acetylchondrosin 6-sulfate + H2O | saturated analogue | Proteus vulgaris NCTC 4636 | acetylchondrosin + sulfate | - |
? | |
| 3.1.6.10 | N-acetyl-D-galactosamine 4,6-disulfate + H2O | - |
Proteus vulgaris | N-acetyl-D-galactosamine 4-sulfate + sulfate | - |
? | |
| 3.1.6.10 | N-acetyl-D-galactosamine 4,6-disulfate + H2O | - |
Proteus vulgaris NCTC 4636 | N-acetyl-D-galactosamine 4-sulfate + sulfate | - |
? | |
| 4.2.2.21 | chondroitin | - |
Pedobacter heparinus | ? | - |
? | |
| 4.2.2.21 | chondroitin | reaction is less efficient than for chondroitin sulfates | Proteus vulgaris | ? | - |
? | |
| 4.2.2.21 | chondroitin sulfate A | - |
Proteus vulgaris | DELTA4,5-unsaturated chondroitin sulfate A disaccharide | - |
? | |
| 4.2.2.21 | chondroitin sulfate A | - |
Pedobacter heparinus | DELTA4,5-unsaturated chondroitin sulfate A disaccharide | - |
? | |
| 4.2.2.21 | chondroitin sulfate B | - |
Proteus vulgaris | DELTA4,5-unsaturated chondroitin sulfate B disaccharide | - |
? | |
| 4.2.2.21 | chondroitin sulfate C | - |
Proteus vulgaris | DELTA4,5-unsaturated chondroitin sulfate C disaccharide | - |
? | |
| 4.2.2.21 | chondroitin sulfate C | - |
Pedobacter heparinus | DELTA4,5-unsaturated chondroitin sulfate C disaccharide | - |
? | |
| 4.2.2.21 | hyaluronic acid | - |
Pedobacter heparinus | ? | - |
? | |
| 4.2.2.21 | hyaluronic acid | bad substrate | Proteus vulgaris | ? | - |
? | |
| 4.2.2.21 | additional information | no substrate: chondroitin sulfate B, keratosulfate, heparin, or heparitin sulfate | Pedobacter heparinus | ? | - |
? | |
| 4.2.2.21 | additional information | no substrate: keratosulfate, heparin, or heparitin sulfate | Proteus vulgaris | ? | - |
? | |
| 4.2.2.21 | additional information | no substrate: keratosulfate, heparin, or heparitin sulfate | Proteus vulgaris NCTC 4636 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.21 | monomer | 1 * 150000 or slightly lower, SDS-PAGE | Proteus vulgaris |
| 4.2.2.21 | monomer | 1 * 150000 or slightly lower, SDS-PAGE | Pedobacter heparinus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.21 | chondroitinase ABC | - |
Proteus vulgaris |
| 4.2.2.21 | chondroitinase AC | - |
Pedobacter heparinus |
| 4.2.2.21 | chondroitinase-ABC | - |
Proteus vulgaris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.21 | 37 | - |
- |
Proteus vulgaris |
| 4.2.2.21 | 37 | - |
- |
Pedobacter heparinus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.10 | 7.5 | - |
- |
Proteus vulgaris |
| 4.2.2.21 | 5 | - |
substrate hyaluronic acid | Pedobacter heparinus |
| 4.2.2.21 | 6 | - |
substrate chondroitin | Pedobacter heparinus |
| 4.2.2.21 | 6.2 | - |
substrate chondroitin | Proteus vulgaris |
| 4.2.2.21 | 6.6 | - |
substrate chondroitin sulfate A, or C | Pedobacter heparinus |
| 4.2.2.21 | 6.8 | - |
substrate hyaluronic acid | Proteus vulgaris |
| 4.2.2.21 | 8 | - |
substrate chondroitin sulfate A, B or C | Proteus vulgaris |
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