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Literature summary extracted from

  • Michelacci, Y.M.; Horton, D.S.P.Q.; Poblacion, C.A.
    Isolation and characterization of an induced chondroitinase ABC from Flavobacterium heparinum (1987), Biochim. Biophys. Acta, 923, 291-301.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.21 Ca2+ 10 mM, partial inhibition Pedobacter heparinus
4.2.2.21 Fe3+ 10 mM, complete inhibition Pedobacter heparinus
4.2.2.21 Mn2+ 10 mM, partial inhibition Pedobacter heparinus
4.2.2.21 phosphate 10 mM, 10% residual activity Pedobacter heparinus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.21 Pedobacter heparinus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.21 1.45
-
 30°C, pH 7.5 Pedobacter heparinus

Storage Stability

EC Number Storage Stability Organism
4.2.2.21 -20°C, 12 months, no loss of activity Pedobacter heparinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 chondroitin 4-sulfate
-
 Pedobacter heparinus unsaturated chondroitin 4-sulfate disaccharide
-
 ?
4.2.2.21 chondroitin 6-sulfate
-
 Pedobacter heparinus unsaturated chondroitin 6-sulfate disaccharide
-
 ?
4.2.2.21 dermatan sulfate
-
 Pedobacter heparinus unsaturated dermatan sulfate disaccharide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.21 30
-
-
 Pedobacter heparinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.21 37
-
 15% residual activity Pedobacter heparinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.21 6 7.5
-
 Pedobacter heparinus