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Literature summary extracted from

  • Elyakova, L.A.; Favorov, V.V.
    Isolation and certain properties of alginate lyase VI from the mollusk Littorina sp. (1974), Biochim. Biophys. Acta, 358, 341-354.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Cd2+
-
 Littorina sp.
4.2.2.3 Cu2+
-
 Littorina sp.
4.2.2.3 dodecylsulfate
-
 Littorina sp.
4.2.2.3 Hg2+
-
 Littorina sp.
4.2.2.3 PCMB
-
 Littorina sp.
4.2.2.3 Zn2+
-
 Littorina sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 0.19
-
 alginate
-
 Littorina sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ba2+ 1 mM, 55% activation Littorina sp.
4.2.2.3 Co2+ 1 mM, 80% activation Littorina sp.
4.2.2.3 Cs+ 1 mM, 40% activation Littorina sp.
4.2.2.3 K+ 1 mM, 15% activation Littorina sp.
4.2.2.3 Li+ 1 mM, 12% activation Littorina sp.
4.2.2.3 Mg2+ 1 mM, 40% activation Littorina sp.
4.2.2.3 NaN3 1 mM, 20% activation Littorina sp.
4.2.2.3 NH4+ 1 mM, 12% activation Littorina sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 40000
-
 gel filtration Littorina sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Littorina sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Littorina sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.3 hepatopancreas
-
 Littorina sp.
-

Storage Stability

EC Number Storage Stability Organism
4.2.2.3 -20°C, 3 months, stable Littorina sp.
4.2.2.3 4°C, in presence of sodium azide, 10 days, 10% loss of activity Littorina sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate preferential splitting of the glycosidic bond between two mannuronide residues in the alginic acid Littorina sp. oligouronides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 pH 5.6, 1 h, stable Littorina sp.
4.2.2.3 50
-
 1 h, complete inactivation Littorina sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 5.6
-
-
 Littorina sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5 7 pH 5.0: about 40% of maximal activity, pH 7.0: about 25% of maximal activity Littorina sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 4 8 stable Littorina sp.
4.2.2.3 4 10 37°C, 3 h, about 40% loss of activity at pH 4.0, about 30% loss of activity, completely stable at pH 5.0 Littorina sp.