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Literature summary extracted from

  • Nakagawa, A.; Ozaki, T.; Chubachi, K.; Hosoyama, T.; Okubo, T.; Iyobe, S.; Suzuki, T.
    An effective method for isolating alginate lyase-producing Bacillus sp. ATB-1015 strain and purification and characterization of the lyase (1998), J. Appl. Microbiol., 84, 328-335.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Cu2+ CuCl2 Bacillus sp. (in: Bacteria)
4.2.2.3 EDTA
-
 Bacillus sp. (in: Bacteria)
4.2.2.11 EDTA complete inhibition at 1 mM concentration, almost completely recovered activity by the addition of 2 mM CaCl2 or partially recovered by the addition of CoCl2 or MnCl2, suggesting that the enzyme conformation is bivalent-cation dependent Bacillus sp. (in: Bacteria)
4.2.2.11 Guanidinium chloride concentrations above 3 M cause significant loss of activity Bacillus sp. (in: Bacteria)
4.2.2.11 additional information 10 mM SDS, protein denaturants or 1 mM urea do not affect enzyme activity Bacillus sp. (in: Bacteria)
4.2.2.11 Urea 36% inhibition in the presence of 8 M urea Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.11 Cu2+ 2 mM, 66% inhibition Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 39000
-
 gel filtration Bacillus sp. (in: Bacteria)
4.2.2.3 41000
-
 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)
4.2.2.11 39000
-
 Sephacryl S-200 HR column chromatography Bacillus sp. (in: Bacteria)
4.2.2.11 41000
-
 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.11 poly-alpha-L-guluronic acid Bacillus sp. (in: Bacteria)
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid Bacillus sp. (in: Bacteria) ATB-1015
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Bacillus sp. (in: Bacteria)
-
 ATB-1015
-
4.2.2.3 Bacillus sp. (in: Bacteria) ATB-1015
-
 ATB-1015
-
4.2.2.11 Bacillus sp. (in: Bacteria)
-
 ATB-1015 strain
-
4.2.2.11 Bacillus sp. (in: Bacteria) ATB-1015
-
 ATB-1015 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Bacillus sp. (in: Bacteria)
4.2.2.11 ammonium sulfate precipitation, gel filtration and anion-exchange chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Bacillus sp. (in: Bacteria) oligouronides
-
 ?
4.2.2.3 alginate
-
 Bacillus sp. (in: Bacteria) ATB-1015 oligouronides
-
 ?
4.2.2.3 polymannuronate
-
 Bacillus sp. (in: Bacteria) 4,5-unsaturated oligomannuronic acids
-
 ?
4.2.2.3 polymannuronate
-
 Bacillus sp. (in: Bacteria) ATB-1015 4,5-unsaturated oligomannuronic acids
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ATB-1015 ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ATB-1015 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-beta1,4-D-mannuronan seems to have slight activity on poly-mannuronan Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.11 poly-beta1,4-D-mannuronan seems to have slight activity on poly-mannuronan Bacillus sp. (in: Bacteria) ATB-1015 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)
4.2.2.11 monomer 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 35 40
-
 Bacillus sp. (in: Bacteria)
4.2.2.11 37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 20 60 20°C: about 55% of maximal activity, 60°C: about 50% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 20
-
 30 min, 40% loss of activity Bacillus sp. (in: Bacteria)
4.2.2.3 60
-
 30 min, 50% loss of activity Bacillus sp. (in: Bacteria)
4.2.2.11 60
-
 50% of the activity remains after treatment at this temperature for 30 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7 8
-
 Bacillus sp. (in: Bacteria)
4.2.2.11 7.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 4 9 pH 4.0: about 40% of maximal activity, pH 9.0: about 70% of maximal activity Bacillus sp. (in: Bacteria)
4.2.2.11 7 8 about 70% activity retained at pH 9.0 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5
-
 relatively stable at alkaline pH, unstable below pH 5.0 Bacillus sp. (in: Bacteria)
4.2.2.11 5
-
 relatively stable in alkaline pH and unstable in a range outside acidic pH 5.0 Bacillus sp. (in: Bacteria)