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Literature summary extracted from

  • Van Houdenhoven, F.E.A.
    Studies on pectin lyase (1975), Mededelingen Landbouwhogeschool, Communications Agricultural University, 75-13, 1-100.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.2.10 high ionic strength or high salt concentration appear to be essential for enzyme stability Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 acetate
-
Aspergillus niger
4.2.2.10 formate
-
Aspergillus niger
4.2.2.10 iso-butyrate
-
Aspergillus niger
4.2.2.10 n-Butyrate
-
Aspergillus niger
4.2.2.10 propionate
-
Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.10 additional information
-
additional information
-
Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.10 Ca2+ optimal concentration above pH 4.5: 50 mM. Optimal cocentration below pH 4.5: 30 mM Aspergillus niger
4.2.2.10 Na+ optimal concentration above pH 4.5: 300 mM. Optimal concentration below pH 4.5: 100 mM Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 35400 36800 type II enzyme, gel filtration Aspergillus niger
4.2.2.10 35500 37950 type I enzyme, gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus niger
-
pectin lyase type I and type II
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.10 side-chain modification 2.5% sugar content, based on weight. Type I enzyme contains only mannose residues, 4 mol/mol. Type II enzyme can be separated into two species which are different in their ratio of mannose to glucose residues Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10
-
Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 pectin
-
Aspergillus niger ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.10 monomer 1 * 45050-50150, enzyme type I, SDS-PAGE Aspergillus niger
4.2.2.10 monomer 1 * 43800-49450, enzyme type II, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 Ultrazym-100 Ciba-Geigy Aspergillus niger
4.2.2.10 Ultrazym-20 Ciba-Geigy Aspergillus niger
4.2.2.10 Ultrazym-40 Ciba-Geigy Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.10 additional information
-
additional information
-
Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 additional information
-
a bell shaped pH profile at low substrate concentrations, shifts of the pH optimum to more basic values upon increasing substrate concentrations accompanied by deviation of the profile from a bell shaped curve Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 additional information
-
-
Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 6
-
inactivation above pH 6 Aspergillus niger