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Literature summary extracted from

  • Morley, C.G.D.; Stadtman, T.C.
    Studies on the fermentation of D-alpha-lysine. Purification and properties of an adenosine triphosphate regulated B12-coenzyme-dependent D-alpha-lysine mutase complex from Clostridium sticklandii (1970), Biochemistry, 9, 4890-4900.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.3.3 mercaptan required Acetoanaerobium sticklandii

General Stability

EC Number General Stability Organism
5.4.3.3 inactivated by irradiation Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.3.3 1,4-diaminobutane
-
 Acetoanaerobium sticklandii
5.4.3.3 2-amino-n-butanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 2-amino-n-pentanoic acid
-
 Acetoanaerobium sticklandii
5.4.3.3 2-aminoisobutanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 3-amino-n-butanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 3-aminoisobutanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 4-amino-n-butanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 6-amino-n-hexanoic acid
-
 Acetoanaerobium sticklandii
5.4.3.3 acetate
-
 Acetoanaerobium sticklandii
5.4.3.3 Butyrate
-
 Acetoanaerobium sticklandii
5.4.3.3 DL-citrulline
-
 Acetoanaerobium sticklandii
5.4.3.3 DL-delta-hydroxylysine
-
 Acetoanaerobium sticklandii
5.4.3.3 DL-epsilon-N-Acetyllysine
-
 Acetoanaerobium sticklandii
5.4.3.3 DL-Pro
-
 Acetoanaerobium sticklandii
5.4.3.3 iodoacetamide
-
 Acetoanaerobium sticklandii
5.4.3.3 L-2,4-diamino-n-butyrate
-
 Acetoanaerobium sticklandii
5.4.3.3 L-beta-Lysine
-
 Acetoanaerobium sticklandii
5.4.3.3 L-Ile
-
 Acetoanaerobium sticklandii
5.4.3.3 L-Orn
-
 Acetoanaerobium sticklandii
5.4.3.3 n-Hexanoate
-
 Acetoanaerobium sticklandii
5.4.3.3 S-aminoethylcysteine
-
 Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.3.3 K+ stimulation by monovalent cations, K+ or NH4+ Acetoanaerobium sticklandii
5.4.3.3 Mg2+ stimulates Acetoanaerobium sticklandii
5.4.3.3 NH4+ stimulation by monovalent cations, K+ or NH4+ Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.3.3 250000
-
 gel filtration Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.3 D-alpha-Lysine Acetoanaerobium sticklandii enzyme of lysine fermentation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.3 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.3
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.3.3 1.08
-
-
 Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
5.4.3.3 -15°C, Tris buffer, pH 8.5-9.0, protein concentration of no less than 3 mg/ml, 20-40% loss of activity after 1 month Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.3 D-alpha-Lysine
-
 Acetoanaerobium sticklandii 2,5-Diaminohexanoate
-
 ?
5.4.3.3 D-alpha-Lysine enzyme of lysine fermentation pathway Acetoanaerobium sticklandii ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.3.3 9 9.2 Tris/HCl buffer Acetoanaerobium sticklandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.3.3 8 10.5 pH 8.0: about 55% of maximal activity, pH 10.5: about 65% of maximal activity, no activity at pH 7 Acetoanaerobium sticklandii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.3.3 4
-
 dissociation of complex Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.3 ATP or phosphonic acid analogs, stimulates, allosteric effector Acetoanaerobium sticklandii
5.4.3.3 Cobalamin required Acetoanaerobium sticklandii
5.4.3.3 pyridoxal 5'-phosphate required Acetoanaerobium sticklandii