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Literature summary extracted from

  • Schlemmer, A.F.; Ware, C.F.; Keen, N.T.
    Purification and characterization of a pectin lyase produced by Pseudomonas fluorescens W51 (1987), J. Bacteriol., 169, 4493-4498.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.2.2.10 20% loss of activity after overnight dialysis against 10 mM potassium phosphate, pH 7.5. 50% loss of activity after overnight dialysis against 10 mM Tris-HCl, pH 7.5. Complete loss of activity after dialysis against water Pseudomonas fluorescens
4.2.2.10 freezing and thawing causes 20% inactivation Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.10 additional information
-
 additional information Km for citrus pectin: 3.2 mg/ml Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 32000
-
 x * 32000, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.10 additional information Pseudomonas fluorescens constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens ?
-
 ?
4.2.2.10 additional information Pseudomonas fluorescens W51 constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Pseudomonas fluorescens
-
 W51
-
4.2.2.10 Pseudomonas fluorescens W51
-
 W51
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 additional information
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 additional information constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens Pseudomonas fluorescens ?
-
 ?
4.2.2.10 additional information constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens Pseudomonas fluorescens W51 ?
-
 ?
4.2.2.10 pectin
-
 Pseudomonas fluorescens ?
-
 ?
4.2.2.10 pectin
-
 Pseudomonas fluorescens W51 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.10 ? x * 32000, SDS-PAGE Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 20
-
 room temperature, 5 min, 30% loss of activity Pseudomonas fluorescens
4.2.2.10 36
-
 10 min, 30% loss of activity Pseudomonas fluorescens
4.2.2.10 42
-
 5 min, 30% loss of activity Pseudomonas fluorescens
4.2.2.10 68
-
 pH 7.8, 5 min, 97% loss of activity Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 8 8.5 citrus pectin, Tris-HCl buffer Pseudomonas fluorescens