EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.9 | Cu2+ | strong | Methanococcus aeolicus | |
4.2.1.9 | KCN | - |
Methanococcus aeolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.9 | 2.8 | - |
2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.9 | Co2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
4.2.1.9 | Fe2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
4.2.1.9 | Mg2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
4.2.1.9 | Mn2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
4.2.1.9 | Ni2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 150000 | - |
gel filtration | Methanococcus aeolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-isoleucine + 2-oxoglutarate | Methanococcus aeolicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | Methanococcus maripaludis | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | Methanococcus voltae | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | Methanococcus aeolicus | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | Methanococcus maripaludis | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | Methanococcus voltae | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | Methanococcus aeolicus | last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | Methanococcus aeolicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | Methanococcus maripaludis | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | Methanococcus voltae | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
4.2.1.9 | 2,3-dihydroxyisovalerate | Methanococcus aeolicus | - |
? | - |
? | |
4.2.1.9 | 2,3-dihydroxyisovalerate | Methanococcus maripaludis | - |
? | - |
? | |
4.2.1.9 | 2,3-dihydroxyisovalerate | Methanococcus voltae | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.42 | Methanococcus aeolicus | - |
- |
- |
2.6.1.42 | Methanococcus maripaludis | - |
JJ, DSM 2067 | - |
2.6.1.42 | Methanococcus voltae | - |
PS, DSM 1537 | - |
4.2.1.9 | Methanococcus aeolicus | - |
- |
- |
4.2.1.9 | Methanococcus maripaludis | - |
- |
- |
4.2.1.9 | Methanococcus voltae | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.9 | 5% O2, at 25°C, half-life: less than 6 min, under anaerobic conditions the enzyme is stable for more than 8 days | Methanococcus aeolicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.42 | partial | Methanococcus aeolicus |
2.6.1.42 | partial | Methanococcus maripaludis |
2.6.1.42 | partial | Methanococcus voltae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 0.006 | - |
- |
Methanococcus aeolicus |
4.2.1.9 | 1.63 | - |
- |
Methanococcus aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-isoleucine + 2-oxo-3-methylpentanoate | - |
Methanococcus aeolicus | 2-oxoisohexanoate + L-isoleucine | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxo-isopentanoate | - |
Methanococcus aeolicus | 2-oxoisohexanoate + L-valine | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Methanococcus maripaludis | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Methanococcus voltae | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-isoleucine + 2-oxoisohexanoate | - |
Methanococcus aeolicus | 3-methyl-2-oxopentanoate + L-leucine | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Methanococcus aeolicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Methanococcus maripaludis | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | - |
Methanococcus voltae | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | Methanococcus aeolicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | Methanococcus maripaludis | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine | Methanococcus voltae | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-leucine + 2-oxoglutarate | last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine | Methanococcus aeolicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Methanococcus aeolicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Methanococcus maripaludis | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
2.6.1.42 | L-valine + 2-oxoglutarate | - |
Methanococcus voltae | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus aeolicus | 2-oxoisovalerate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus maripaludis | 2-oxoisovalerate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus voltae | 2-oxoisovalerate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus aeolicus | 3-methyl-2-oxopentanoate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus maripaludis | 3-methyl-2-oxopentanoate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus voltae | 3-methyl-2-oxopentanoate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus | ? | - |
? | |
4.2.1.9 | 2,3-dihydroxyisovalerate | - |
Methanococcus maripaludis | ? | - |
? | |
4.2.1.9 | 2,3-dihydroxyisovalerate | - |
Methanococcus voltae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.42 | BcaT | - |
Methanococcus aeolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.9 | 7.5 | - |
- |
Methanococcus aeolicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.9 | 6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity | Methanococcus aeolicus |