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Literature summary extracted from

  • Xing, R.; Whitman, W.B.
    Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp (1991), J. Bacteriol., 173, 2086-2092.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.9 Cu2+ strong Methanococcus aeolicus
4.2.1.9 KCN
-
Methanococcus aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.9 2.8
-
2,3-dihydroxyisovalerate
-
Methanococcus aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.9 Co2+ divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective Methanococcus aeolicus
4.2.1.9 Fe2+ divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective Methanococcus aeolicus
4.2.1.9 Mg2+ divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective Methanococcus aeolicus
4.2.1.9 Mn2+ divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective Methanococcus aeolicus
4.2.1.9 Ni2+ divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective Methanococcus aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 150000
-
gel filtration Methanococcus aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate Methanococcus aeolicus
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate Methanococcus maripaludis
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate Methanococcus voltae
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus aeolicus biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus maripaludis biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus voltae biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate Methanococcus aeolicus last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Methanococcus aeolicus
-
3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Methanococcus maripaludis
-
3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate Methanococcus voltae
-
3-methyl-2-oxobutanoate + L-glutamate
-
r
4.2.1.9 2,3-dihydroxyisovalerate Methanococcus aeolicus
-
?
-
?
4.2.1.9 2,3-dihydroxyisovalerate Methanococcus maripaludis
-
?
-
?
4.2.1.9 2,3-dihydroxyisovalerate Methanococcus voltae
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Methanococcus aeolicus
-
-
-
2.6.1.42 Methanococcus maripaludis
-
JJ, DSM 2067
-
2.6.1.42 Methanococcus voltae
-
PS, DSM 1537
-
4.2.1.9 Methanococcus aeolicus
-
-
-
4.2.1.9 Methanococcus maripaludis
-
-
-
4.2.1.9 Methanococcus voltae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.9 5% O2, at 25°C, half-life: less than 6 min, under anaerobic conditions the enzyme is stable for more than 8 days Methanococcus aeolicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 partial Methanococcus aeolicus
2.6.1.42 partial Methanococcus maripaludis
2.6.1.42 partial Methanococcus voltae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 0.006
-
-
Methanococcus aeolicus
4.2.1.9 1.63
-
-
Methanococcus aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxo-3-methylpentanoate
-
Methanococcus aeolicus 2-oxoisohexanoate + L-isoleucine
-
r
2.6.1.42 L-isoleucine + 2-oxo-isopentanoate
-
Methanococcus aeolicus 2-oxoisohexanoate + L-valine
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Methanococcus aeolicus 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Methanococcus maripaludis 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Methanococcus voltae 3-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-isoleucine + 2-oxoisohexanoate
-
Methanococcus aeolicus 3-methyl-2-oxopentanoate + L-leucine
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Methanococcus aeolicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Methanococcus maripaludis 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate
-
Methanococcus voltae 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine Methanococcus aeolicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine Methanococcus maripaludis 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate biosynthesis of the branched-chain amino acids L-valine, L-isoleucine and L-leucine Methanococcus voltae 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-leucine + 2-oxoglutarate last step in the biosynthesis of the branched-chain amino acids L-isoleucine, L-valine and L-leucine Methanococcus aeolicus 4-methyl-2-oxopentanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Methanococcus aeolicus 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Methanococcus maripaludis 3-methyl-2-oxobutanoate + L-glutamate
-
r
2.6.1.42 L-valine + 2-oxoglutarate
-
Methanococcus voltae 3-methyl-2-oxobutanoate + L-glutamate
-
r
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Methanococcus aeolicus 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Methanococcus maripaludis 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Methanococcus voltae 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Methanococcus aeolicus 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Methanococcus maripaludis 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Methanococcus voltae 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxyisovalerate
-
Methanococcus aeolicus ?
-
?
4.2.1.9 2,3-dihydroxyisovalerate
-
Methanococcus maripaludis ?
-
?
4.2.1.9 2,3-dihydroxyisovalerate
-
Methanococcus voltae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
Methanococcus aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.9 7.5
-
-
Methanococcus aeolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.9 6 9 pH 6.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity Methanococcus aeolicus