EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.9 | additional information | enzyme from iv-1 mutant strain 332 differs from the wild type enzyme with respect to heat-inactivation, pH-dependence, and kinetics. The altered structure of the mutant enzyme is the basic defect hindering its incorporation into mitochondria | Neurospora crassa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.9 | 3.7 | - |
2,3-dihydroxy-3-methylpentanoate | wild-type enzyme | Neurospora crassa | |
4.2.1.9 | 8.5 | - |
2,3-dihydroxy-3-methylpentanoate | mutant enzyme 332 | Neurospora crassa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.9 | Neurospora crassa | - |
wild type strain KJT 1960 and iv-mutant strain 332 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.9 | 2,3-dihydroxy-3-methylbutanoate | - |
Neurospora crassa | 2-oxoisovalerate + H2O | - |
? | |
4.2.1.9 | 2,3-dihydroxy-3-methylpentanoate | - |
Neurospora crassa | 3-methyl-2-oxopentanoate + H2O | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.9 | 46.5 | - |
20 min, 50% loss of activity, mutant strain 332 | Neurospora crassa |
4.2.1.9 | 56 | - |
20 min, 50% loss of activity, wild type | Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.9 | 7.8 | - |
2,3-dihydroxy-3-methylpentanoate | Neurospora crassa |
4.2.1.9 | 7.8 | - |
mutant strain 332 | Neurospora crassa |
4.2.1.9 | 8.2 | - |
2,3-dihydroxy-3-methylpentanoate, wild-type strain | Neurospora crassa |