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Literature summary extracted from

  • Altmiller, D.H.
    Neurospora mutants with mitochondria deficient in dihydroxy acid dehydratase. Properties of dihydroxy acid dehydratase from mutant strain 332 (1972), Biochem. Biophys. Res. Commun., 49, 1000-1006.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.9 additional information enzyme from iv-1 mutant strain 332 differs from the wild type enzyme with respect to heat-inactivation, pH-dependence, and kinetics. The altered structure of the mutant enzyme is the basic defect hindering its incorporation into mitochondria Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.9 3.7
-
2,3-dihydroxy-3-methylpentanoate wild-type enzyme Neurospora crassa
4.2.1.9 8.5
-
2,3-dihydroxy-3-methylpentanoate mutant enzyme 332 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.9 Neurospora crassa
-
wild type strain KJT 1960 and iv-mutant strain 332
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Neurospora crassa 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Neurospora crassa 3-methyl-2-oxopentanoate + H2O
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.9 46.5
-
20 min, 50% loss of activity, mutant strain 332 Neurospora crassa
4.2.1.9 56
-
20 min, 50% loss of activity, wild type Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.9 7.8
-
2,3-dihydroxy-3-methylpentanoate Neurospora crassa
4.2.1.9 7.8
-
mutant strain 332 Neurospora crassa
4.2.1.9 8.2
-
2,3-dihydroxy-3-methylpentanoate, wild-type strain Neurospora crassa