Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Myers, J.W.
    Dihydroxy acid dehydrase: an enzyme involved in the biosynthesis of isoleucine and valine (1961), J. Biol. Chem., 236, 1414-1418.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.9 Cu2+ CuSO4 Escherichia coli
4.2.1.9 EDTA
-
Escherichia coli
4.2.1.9 NaF
-
Escherichia coli
4.2.1.9 PCMB
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.9 Fe2+ divalent metal ion required, Fe2+ is most effective Escherichia coli
4.2.1.9 Mg2+ divalent metal ion required, less effective than Fe2+ Escherichia coli
4.2.1.9 Mn2+ divalent metal ion required, less effective than Fe2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.9 2,3-dihydroxy-3-methylpentanoate Escherichia coli
-
?
-
?
4.2.1.9 2,3-dihydroxyisovalerate Escherichia coli
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.9 Escherichia coli
-
K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.9 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.9 0.416
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Escherichia coli 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Escherichia coli 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate
-
Escherichia coli ?
-
?
4.2.1.9 2,3-dihydroxyisovalerate
-
Escherichia coli ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.9 7.8
-
2,3-dihydroxy-3-methylpentanoate Escherichia coli
4.2.1.9 7.9
-
2,3-dihydroxyisovalerate Escherichia coli