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Literature summary extracted from

  • Stransky, H.; Amberger, A.
    Isolierung und Eigenschaften einer Cyanamid-hydratase (E.C.-Gruppe 4.2.1.) aus Myrothecium verrucaria (1973), Z. Pflanzenphysiol., 70, 74-87.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.69 Co2+ above 0.01 M Albifimbria verrucaria
4.2.1.69 Cu2+ above 0.01 M Albifimbria verrucaria
4.2.1.69 Fe2+ above 0.01 M Albifimbria verrucaria
4.2.1.69 Mg2+ above 0.01 M Albifimbria verrucaria
4.2.1.69 Mn2+ above 0.01 M Albifimbria verrucaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.69 0.0037
-
 cyanamide
-
 Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.69 Mn2+ activates Albifimbria verrucaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.69 cyanamide + H2O Albifimbria verrucaria no constitutive enzyme, induced by addition of cyanamide to growth medium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.69 Albifimbria verrucaria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.69 partial Albifimbria verrucaria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.69 73.65
-
-
 Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.69 cyanamide + H2O
-
 Albifimbria verrucaria urea
-
 ir
4.2.1.69 cyanamide + H2O no constitutive enzyme, induced by addition of cyanamide to growth medium Albifimbria verrucaria ?
-
 ?
4.2.1.69 additional information not: Arg, Orn, urea, guanidine, guanidino-urea, creatin, creatinin, guanidinoacetate Albifimbria verrucaria ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.69 40 55
-
 Albifimbria verrucaria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.69 15 65 less than 50% of maximal activity above and below Albifimbria verrucaria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.69 70
-
 stable at Albifimbria verrucaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.69 7.7
-
-
 Albifimbria verrucaria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.69 6.4 8.5 less than 50% of maximal activity above and below Albifimbria verrucaria