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Literature summary extracted from

  • Veiga, L.A.; Guimaes, M.F.
    L.Fuconate dehydratase: purification and properties of the enzyme from Pullularia pullulans (1991), Arq. Biol. Tecnol., 34, 537-553.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.68 very unstable, not stabilized by MgCl2 or EDTA Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.68 D-arabonate 10 mM, 100% inhibition Aureobasidium pullulans
4.2.1.68 D-arabono-1,5-lactone
-
 Aureobasidium pullulans
4.2.1.68 D-galactonate 10 mM, 100% inhibition Aureobasidium pullulans
4.2.1.68 D-gluconate 10 mM, 100% inhibition Aureobasidium pullulans
4.2.1.68 EDTA 10 mM, 100% inhibition Aureobasidium pullulans
4.2.1.68 HgCl2 0.25 mM, ca. 90% inhibition Aureobasidium pullulans
4.2.1.68 iodoacetate 2.5 mM, 90% inhibition Aureobasidium pullulans
4.2.1.68 L-Rhamnonate 10 mM, 95% inhibition Aureobasidium pullulans
4.2.1.68 N-ethylmaleimide 1 mM, 81% inhibition Aureobasidium pullulans
4.2.1.68 p-chloromercuribenzoate 0.25 mM, 79% inhibition Aureobasidium pullulans
4.2.1.68 ZnSO4 5 mM, 78% inhibition Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.68 0.6
-
 L-fuconate
-
 Aureobasidium pullulans
4.2.1.68 3.3
-
 L-galactonate
-
 Aureobasidium pullulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.68 95000
-
 gel filtration Aureobasidium pullulans
4.2.1.68 104000
-
 sucrose density ultracentifugation Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.68 L-Fuconate Aureobasidium pullulans catabolism of L-fucose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.68 Aureobasidium pullulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.68
-
 Aureobasidium pullulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.68 29.1
-
-
 Aureobasidium pullulans

Storage Stability

EC Number Storage Stability Organism
4.2.1.68 -20°C, 30% loss of activity in 1 month Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.68 L-fuconate
-
 Aureobasidium pullulans 2-dehydro-3-deoxy-L-fuconate + H2O
-
 ?
4.2.1.68 L-Fuconate catabolism of L-fucose Aureobasidium pullulans ?
-
 ?
4.2.1.68 L-galactonate
-
 Aureobasidium pullulans 2-keto-3-deoxy-L-galactonate + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.68 40
-
-
 Aureobasidium pullulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.68 30 50 less than 50% of maximal activity above and below Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.68 6.8 7.4
-
 Aureobasidium pullulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.68 5 9 less than 50% of maximal activity above and below Aureobasidium pullulans