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Literature summary extracted from

  • Webster, F.H.; Lechowich, R.V.
    Partial purification and characterization of dihydrodipicolinic acid synthetase from sporulating Bacillus megaterium (1970), J. Bacteriol., 101, 118-126.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.3.7 2-mercaptoethanol activation Priestia megaterium
4.3.3.7 cysteine activation Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.7 L-lysine no inhibition Priestia megaterium
4.3.3.7 additional information not inhibitory: iodoacetate, iodoacetamide, p-chloromercuribenzoate Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.7 0.46
-
 L-aspartate-4-semialdehyde
-
 Priestia megaterium
4.3.3.7 0.5
-
 pyruvate
-
 Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 partial Priestia megaterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.7 sporulating cell
-
 Priestia megaterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.7 additional information
-
-
 Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
4.3.3.7 -20°C, pH 6.5 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 L-aspartate-4-semialdehyde + pyruvate
-
 Priestia megaterium dihydrodipicolinate + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.3.7 60
-
 inactivation energy: 67300 cal/mol, change in free energy, DELTAF: 22500 cal/mol, change in enthalpy, DELTAH: 66700 cal/mol, change in entropy DELTAS: 133 cal/degree/mol Priestia megaterium
4.3.3.7 75
-
 pyruvate increases the half-life from 2.7 to 18.3 min Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.7 7.7
-
-
 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.3.3.7 6.5 8 pH 6.5, half-life: 5.3 min, pH 8..0, half-life: 20 min Priestia megaterium
4.3.3.7 10.5
-
 maximal thermal stability at this pH Priestia megaterium