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Literature summary extracted from

  • Euverink, G.J.W.; Wolters, D.J.; Dijkhuizen, L.
    Prephenate dehydratase of the actinomycete Amycolatopsis methanolica: purification and characterization of wild-type and deregulated mutant proteins (1995), Biochem. J., 308, 313-320.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.51 L-methionine 1 mM, 1.6fold activation Amycolatopsis methanolica
4.2.1.51 L-tyrosine Ka: 410 mM Amycolatopsis methanolica

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.51 L-phenylalanine 1 mM, 48% inhibition, Ki: 670 mM Amycolatopsis methanolica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.51 34000
-
 4 * 34000, SDS-PAGE Amycolatopsis methanolica
4.2.1.51 150000
-
 gel filtration Amycolatopsis methanolica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.51 prephenate Amycolatopsis methanolica
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Amycolatopsis methanolica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.51
-
 Amycolatopsis methanolica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.51 33.3
-
-
 Amycolatopsis methanolica

Storage Stability

EC Number Storage Stability Organism
4.2.1.51 -20°C, 40% v/v glycerol Amycolatopsis methanolica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
 Amycolatopsis methanolica phenylpyruvate + H2O + CO2
-
 ?
4.2.1.51 prephenate
-
 Amycolatopsis methanolica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.51 tetramer 4 * 34000, SDS-PAGE Amycolatopsis methanolica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.51 6.5 8.5
-
 Amycolatopsis methanolica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.51 670
-
 L-phenylalanine
-
 Amycolatopsis methanolica