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Literature summary extracted from
- Probing the coenzyme and substrate binding events of CDP-D-glucose 4,6-dehydratase: mechanistic implications (1996), Biochemistry, 35, 4721-4731.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.45 | expression in Escherichia coli | Yersinia pseudotuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.45 | H17G | mutant with increased kcat and reduced Km | Yersinia pseudotuberculosis |
| 4.2.1.45 | K21I | mutant with reduced Km and kcat | Yersinia pseudotuberculosis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.45 | 0.00015 | - |
CDP-D-glucose | mutant H17G | Yersinia pseudotuberculosis |  |
| 4.2.1.45 | 0.00024 | - |
CDP-D-glucose | mutant K21I | Yersinia pseudotuberculosis | |
| 4.2.1.45 | 0.0003 | - |
CDP-D-glucose | wild-type | Yersinia pseudotuberculosis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.45 | Yersinia pseudotuberculosis | - |
Pasteurella pseudotuberculosis | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.45 | mutant H17G | Yersinia pseudotuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.45 | 2.78 | - |
mutant H17G | Yersinia pseudotuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.45 | CDP-D-glucose | - |
Yersinia pseudotuberculosis | CDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.45 | 2.33 | - |
CDP-D-glucose | mutant K21I | Yersinia pseudotuberculosis | |
| 4.2.1.45 | 3.69 | - |
CDP-D-glucose | wild-type enzyme | Yersinia pseudotuberculosis | |
| 4.2.1.45 | 4.24 | - |
CDP-D-glucose | mutant H17G | Yersinia pseudotuberculosis | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.45 | NAD+ | two binding sites per enzyme molecule | Yersinia pseudotuberculosis | |
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