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Literature summary extracted from
- D-glucarate dehydratase (1966), Methods Enzymol., 9, 660-665.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.40 | Co2+ | high concentrations | Escherichia coli |  |
| 4.2.1.40 | D-idaric acid | - |
Escherichia coli | |
| 4.2.1.40 | EDTA | - |
Escherichia coli | |
| 4.2.1.40 | galactaric acid | - |
Escherichia coli | |
| 4.2.1.40 | Mn2+ | high concentrations | Escherichia coli | |
| 4.2.1.40 | p-chloromercuribenzene sulfonate | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.40 | 0.8 | - |
D-glucarate | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.40 | Mg2+ | - |
Klebsiella aerogenes | |
| 4.2.1.40 | Mg2+ | 80 mM optimal | Escherichia coli | |
| 4.2.1.40 | Mn2+ | less than one-third as effective as Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.40 | D-Glucarate | Bacillus subtilis | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Escherichia coli | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Priestia megaterium | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Klebsiella aerogenes | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Klebsiella pneumoniae | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Pectobacterium carotovorum | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Citrobacter freundii | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | Paracolobactrum sp. | first step in D-glucarate metabolism in bacteria | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.40 | Bacillus subtilis | - |
- |
- |
| 4.2.1.40 | Citrobacter freundii | - |
- |
- |
| 4.2.1.40 | Escherichia coli | - |
- |
- |
| 4.2.1.40 | Klebsiella aerogenes | - |
- |
- |
| 4.2.1.40 | Klebsiella pneumoniae | - |
- |
- |
| 4.2.1.40 | Paracolobactrum sp. | - |
- |
- |
| 4.2.1.40 | Pectobacterium carotovorum | - |
- |
- |
| 4.2.1.40 | Priestia megaterium | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.40 | 20.1 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.40 | D-glucarate | - |
Bacillus subtilis | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Priestia megaterium | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Klebsiella pneumoniae | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Pectobacterium carotovorum | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Citrobacter freundii | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Paracolobactrum sp. | 3-deoxy-L-threo-2-hexulosarate + H2O | - |
? | |
| 4.2.1.40 | D-glucarate | - |
Escherichia coli | 3-deoxy-L-threo-2-hexulosarate + H2O | i.e. 4-deoxy-5-ketoglucarate | ir | |
| 4.2.1.40 | D-glucarate | - |
Klebsiella aerogenes | 3-deoxy-L-threo-2-hexulosarate + H2O | i.e. 4-deoxy-5-ketoglucarate | ? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Bacillus subtilis | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Escherichia coli | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Priestia megaterium | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Klebsiella aerogenes | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Klebsiella pneumoniae | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Pectobacterium carotovorum | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Citrobacter freundii | ? | - |
? | |
| 4.2.1.40 | D-Glucarate | first step in D-glucarate metabolism in bacteria | Paracolobactrum sp. | ? | - |
? | |
| 4.2.1.40 | L-idarate | - |
Escherichia coli | 3-deoxy-L-threo-2-hexulosarate | i.e. 4-deoxy-5-ketoglucarate | ? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.40 | 7.2 | - |
Tris-maleate buffer | Escherichia coli |
| 4.2.1.40 | 7.5 | - |
barbital buffer | Escherichia coli |
| 4.2.1.40 | 7.5 | 8.5 | Tris-HCl buffer | Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.40 | 5 | 6 | moderately stable | Escherichia coli |
| 4.2.1.40 | 7.5 | - |
most stable near | Escherichia coli |
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