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Literature summary extracted from

  • Jeffcoat, R.
    Chemical modification and kinetic studies on glucarate hydro-lyase from a species of Pseudomonas A (1972), Eur. J. Biochem., 25, 515-523.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.40 (+)-tartrate 2.5 mM, 58% inhibition Pseudomonas sp.
4.2.1.40 (-)-tartrate 2.5 mM, 5% inhibition Pseudomonas sp.
4.2.1.40 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Pseudomonas sp.
4.2.1.40 5-Amino-1H-tetrazole
-
 Pseudomonas sp.
4.2.1.40 EDTA 1 mM, 94.5% inhibition Pseudomonas sp.
4.2.1.40 meso-tartrate 2.5 mM, 31% inhibition Pseudomonas sp.
4.2.1.40 p-chloromercuribenzene sulfonate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.40 1.42
-
 D-glucarate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.40 Mg2+ required Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.40 Pseudomonas sp.
-
 A
-
4.2.1.40 Pseudomonas sp. A
-
 A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.40 partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.40 3.7
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.40 D-glucarate
-
 Pseudomonas sp. 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?
4.2.1.40 D-glucarate
-
 Pseudomonas sp. A 3-deoxy-L-threo-2-hexulosarate + H2O i.e. 4-deoxy-5-ketoglucarate ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.40 8
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.40 5.5 9.5
-
 Pseudomonas sp.