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Literature summary extracted from

  • Seyfried, M; Daniel, R.; Gottschalk, G.
    Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii (1996), J. Bacteriol., 178, 5793-5796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 expression in Escherichia coli Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 additional information
-
 additional information
-
 Klebsiella pneumoniae
4.2.1.30 additional information
-
 additional information
-
 Limosilactobacillus reuteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 16121
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
4.2.1.30 21487
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
4.2.1.30 60433
-
 alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii
4.2.1.30 198000
-
 gel filtration, native enzyme Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 glycerol Citrobacter freundii
-
 ?
-
 ?
4.2.1.30 glycerol Limosilactobacillus reuteri
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Citrobacter freundii
-
-
-
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Limosilactobacillus reuteri
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Citrobacter freundii 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Limosilactobacillus reuteri 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 glycerol
-
 Citrobacter freundii ?
-
 ?
4.2.1.30 glycerol
-
 Limosilactobacillus reuteri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 hexamer alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Citrobacter freundii
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Limosilactobacillus reuteri