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Literature summary extracted from

  • Tobimatsu, T.; Azuma, M.; Matsubara, H.; Takatori, H.; Niida, T.; Nishimoto, K.; Satoh, H.; Hayashi, R.; Toraya, T.
    Cloning, sequencing, and high level expression of the genes encoding adenosylcobalamin-dependent glycerol dehydrase of Klebsiella pneumoniae (1996), J. Biol. Chem., 271, 22352-22357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 expression in Escherichia coli Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 21355
-
 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae
4.2.1.30 22000
-
 1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae
4.2.1.30 60659
-
 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae
4.2.1.30 61000
-
 1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 additional information Klebsiella pneumoniae enzyme production when grown anaerobically in a glycerol medium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
 ATCC 25955
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 1.3
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 additional information enzyme production when grown anaerobically in a glycerol medium Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 dimer !1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE Klebsiella pneumoniae
4.2.1.30 dimer 2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 adenosylcobalamin required Klebsiella pneumoniae