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Literature summary extracted from

  • Talarico, T.L.; Dobrogosz, W.J.
    Purification and characterization of glycerol dehydratase from Lactobacillus reuteri (1990), Appl. Environ. Microbiol., 56, 1195-1197.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.2.1.30 preservation of enzyme activity requires the presence of K+ and 1,2-propanediol Limosilactobacillus reuteri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 additional information
-
 additional information
-
 Klebsiella pneumoniae
4.2.1.30 additional information
-
 additional information
-
 Limosilactobacillus reuteri
4.2.1.30 3.3
-
 glycerol in the presence of 0.5 mM coenzyme B12 Limosilactobacillus reuteri
4.2.1.30 7
-
 1,2-propanediol in the presence of 0.5 mM coenzyme B12 Limosilactobacillus reuteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 52000
-
 4 * 52000, SDS-PAGE Limosilactobacillus reuteri
4.2.1.30 200000
-
 gel filtration Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 glycerol Levilactobacillus brevis
-
 ?
-
 ?
4.2.1.30 glycerol Lentilactobacillus buchneri
-
 ?
-
 ?
4.2.1.30 glycerol Limosilactobacillus reuteri
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Lentilactobacillus buchneri
-
-
-
4.2.1.30 Levilactobacillus brevis
-
-
-
4.2.1.30 Limosilactobacillus reuteri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30
-
 Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 4
-
-
 Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol + H2O
-
 Limosilactobacillus reuteri propionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Levilactobacillus brevis 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Lentilactobacillus buchneri 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Limosilactobacillus reuteri 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 glycerol
-
 Levilactobacillus brevis ?
-
 ?
4.2.1.30 glycerol
-
 Lentilactobacillus buchneri ?
-
 ?
4.2.1.30 glycerol
-
 Limosilactobacillus reuteri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 tetramer 4 * 52000, SDS-PAGE Limosilactobacillus reuteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.30 7.2
-
-
 Limosilactobacillus reuteri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.30 6.5 8 optimal enzyme activity between Limosilactobacillus reuteri

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Levilactobacillus brevis
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Lentilactobacillus buchneri
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Limosilactobacillus reuteri