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Literature summary extracted from

  • Schneider, Z.; Larsen, E.G.; Jacobson, G.; Johnson, B.C.; Pawelkiewicz, J.
    Purification and properties of glycerol dehydrase (1970), J. Biol. Chem., 245, 3388-3396.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 Ag+ 0.1 mM AgNO3, apoenzyme: 97% loss of activity, inactive enzyme complex with hydroxocobalamin: 19% loss of activity Klebsiella pneumoniae
4.2.1.30 EDTA inhibition of the apoenzyme can be completely reversed by Mg2+ in adequate concentrations. Ca2+, Mn2+, Zn2+ and Co2+ also reverse the EDTA inhibitory effect. Glycerol partially reverses the inhibition. Complete inhibition of the enzyme complex with hydroxocobalamin is reversible with an excess of Mg2+ Klebsiella pneumoniae
4.2.1.30 hexadecyltrimethylammonium bromide 0.14%, complete irreversible inactivation of the apoenzyme Klebsiella pneumoniae
4.2.1.30 Hg2+ 0.1 mM HgCl2, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity Klebsiella pneumoniae
4.2.1.30 additional information
-
Klebsiella pneumoniae
4.2.1.30 p-chloromercuribenzoate 0.1 mM, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 Mg2+ required for the reactivation of the active holoenzyme, optimal concentration: 30 mM Klebsiella pneumoniae
4.2.1.30 SO32- K2SO3, absolute requirement in the reactivation of the inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 188000
-
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
?
-
?
4.2.1.30 glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Klebsiella pneumoniae PZH 572 Warsaw
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 additional information
-
-
Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
4.2.1.30 stable after lyophilization Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
Klebsiella pneumoniae ?
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae PZH 572 Warsaw ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 dimer enzyme dissociates reversibly by action of EDTA into two different very unstable subunits, A and B Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.30 70
-
inactive enzyme complex with hydroxocobalamin: quite stable Klebsiella pneumoniae
4.2.1.30 78
-
K+ stabilizes inactive enzyme complex with hydroxocobalamin Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.30 5 9.5 inactive enzyme complex with hydroxocobalamin, quite stable between pH 5.0 to pH 9.5, below pH 5.0: inactive enzyme complex with hydroxocobalamin dissociates irreversibly, liberating hydroxocobalamin, B12OH Klebsiella pneumoniae
4.2.1.30 8 9 apoenzyme is unstable below pH 8.0 and above pH 9.0 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-dimethylbenzimidazolylcobamide 5'-phosphate i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- Klebsiella pneumoniae