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Literature summary extracted from

  • Kennedy, M.C.; Mende-Mueller, L.; Blondin, G.A.; Beinert, H.
    Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein [published erratum appears in Proc Natl Acad Sci U S A 1993 Mar 15;90(6):2556 (1992), Proc. Natl. Acad. Sci. USA, 89, 11730-11734.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.3 cytosol identical with iron-responsive element binding protein Bos taurus 5829
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.3 Iron cytosolic and mitochondrial isoenzyme require an intact [4Fe-4S] cluster. Mitochondrial aconitase is isolated predominantly in the [3Fe-4S] form (Fe/S ratio of 0.73) and must be activated by the addition of Fe2+. The cytoplasmic aconitase as isolated is about 80% active with a Fe/S ratio of 1.1 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.3 additional information Bos taurus iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Bos taurus
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-
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.3
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 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.3 liver
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 Bos taurus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.3 21.4
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-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 cis-aconitate + H2O
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 Bos taurus isocitrate
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 ?
4.2.1.3 citrate
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 Bos taurus cis-aconitate + H2O
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 ?
4.2.1.3 citrate
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 Bos taurus isocitrate
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 ?
4.2.1.3 isocitrate
-
 Bos taurus cis-aconitate + H2O
-
 ?
4.2.1.3 additional information iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA Bos taurus ?
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 ?