BRENDA - Enzyme Database

Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein [published erratum appears in Proc Natl Acad Sci U S A 1993 Mar 15;90(6):2556

Kennedy, M.C.; Mende-Mueller, L.; Blondin, G.A.; Beinert, H.; Proc. Natl. Acad. Sci. USA 89, 11730-11734 (1992)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
identical with iron-responsive element binding protein
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
cytosolic and mitochondrial isoenzyme require an intact [4Fe-4S] cluster. Mitochondrial aconitase is isolated predominantly in the [3Fe-4S] form (Fe/S ratio of 0.73) and must be activated by the addition of Fe2+. The cytoplasmic aconitase as isolated is about 80% active with a Fe/S ratio of 1.1
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
additional information
Bos taurus
iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
21.4
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate
-
33811
Bos taurus
isocitrate
-
33811
Bos taurus
-
4.2.1.3
citrate
-
33811
Bos taurus
cis-aconitate
-
33811
Bos taurus
-
4.2.1.3
citrate
-
33811
Bos taurus
isocitrate
-
33811
Bos taurus
-
4.2.1.3
isocitrate
-
33811
Bos taurus
cis-aconitate
-
33811
Bos taurus
-
4.2.1.3
additional information
iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA
33811
Bos taurus
?
-
-
-
-
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
identical with iron-responsive element binding protein
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
cytosolic and mitochondrial isoenzyme require an intact [4Fe-4S] cluster. Mitochondrial aconitase is isolated predominantly in the [3Fe-4S] form (Fe/S ratio of 0.73) and must be activated by the addition of Fe2+. The cytoplasmic aconitase as isolated is about 80% active with a Fe/S ratio of 1.1
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
additional information
Bos taurus
iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
21.4
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate
-
33811
Bos taurus
isocitrate
-
33811
Bos taurus
-
4.2.1.3
citrate
-
33811
Bos taurus
cis-aconitate
-
33811
Bos taurus
-
4.2.1.3
citrate
-
33811
Bos taurus
isocitrate
-
33811
Bos taurus
-
4.2.1.3
isocitrate
-
33811
Bos taurus
cis-aconitate
-
33811
Bos taurus
-
4.2.1.3
additional information
iron-responsive element binding protein is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA
33811
Bos taurus
?
-
-
-
-