BRENDA - Enzyme Database

The aconitase of yeast. II. Crystallization and general properties of yeast aconitase

Suzuki, T.; Yamazaki, O.; Nara, K.; Akiyama, S.; Nakao, Y.; Fukuda, H.; J. Biochem. 77, 367-372 (1975)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.3
-
Yarrowia lipolytica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
contains 2.1 mol of iron per mol of enzyme
Yarrowia lipolytica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
68500
-
equilibrium sedimentation
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
additional information
Yarrowia lipolytica
inductively formed in presence of fluoroacetate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Yarrowia lipolytica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
-
Yarrowia lipolytica
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
additional information
-
-
Yarrowia lipolytica
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.3
4°C, 24 h, pH 5.5-9.0, stable
Yarrowia lipolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
33794
Yarrowia lipolytica
cis-aconitate
-
-
-
?
4.2.1.3
additional information
inductively formed in presence of fluoroacetate
33794
Yarrowia lipolytica
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
55
-
-
Yarrowia lipolytica
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
50
-
30 min, stable
Yarrowia lipolytica
4.2.1.3
60
-
10 min, complete inactivation
Yarrowia lipolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
-
Yarrowia lipolytica
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.3
-
Yarrowia lipolytica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
contains 2.1 mol of iron per mol of enzyme
Yarrowia lipolytica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
68500
-
equilibrium sedimentation
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.3
additional information
Yarrowia lipolytica
inductively formed in presence of fluoroacetate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
-
Yarrowia lipolytica
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
additional information
-
-
Yarrowia lipolytica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.3
4°C, 24 h, pH 5.5-9.0, stable
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
33794
Yarrowia lipolytica
cis-aconitate
-
-
-
?
4.2.1.3
additional information
inductively formed in presence of fluoroacetate
33794
Yarrowia lipolytica
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
55
-
-
Yarrowia lipolytica
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
50
-
30 min, stable
Yarrowia lipolytica
4.2.1.3
60
-
10 min, complete inactivation
Yarrowia lipolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
-
Yarrowia lipolytica