BRENDA - Enzyme Database

Studies on two isozymes of aconitase from Bacillus cereus T. I. Partial purification and stability

Agrawal, P.K.; Garg, G.K.; Gollakota, K.G.; Biochem. Biophys. Res. Commun. 67, 645-652 (1975)

Data extracted from this reference:

Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Bacillus cereus
-
early aconitase (isoenzyme I) is present in a culture of 5 h age, late aconitase (isoenzyme II) is present in 12 h age; T
-
4.2.1.3
Bacillus cereus T
-
T
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
partial
Bacillus cereus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
33793
Bacillus cereus
citrate
-
-
-
?
4.2.1.3
isocitrate
-
33793
Bacillus cereus T
citrate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
5
-
15 h, 50% inactivation, isoenzyme I. 15 h, about 65% loss of activity, isoenzyme II
Bacillus cereus
4.2.1.3
30
-
5 h, 50% loss of activity, isoenzyme II. 15 h, 90% loss of activity, isoenzyme I. 15 h, 75% loss of activity, isoenzyme II
Bacillus cereus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
partial
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
33793
Bacillus cereus
citrate
-
-
-
?
4.2.1.3
isocitrate
-
33793
Bacillus cereus T
citrate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
5
-
15 h, 50% inactivation, isoenzyme I. 15 h, about 65% loss of activity, isoenzyme II
Bacillus cereus
4.2.1.3
30
-
5 h, 50% loss of activity, isoenzyme II. 15 h, 90% loss of activity, isoenzyme I. 15 h, 75% loss of activity, isoenzyme II
Bacillus cereus