BRENDA - Enzyme Database

pH profiles and isotope effects for aconitases from Saccharomycopsis lipolytica, beef heart, and beef liver. alpha-Methyl-cis-aconitate and threo-Ds-alpha-methylisocitrate as substrates

Schloss, J.V.; Emptage, M.H.; Cleland, W.W.; Biochemistry 23, 4572-4580 (1984)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
pH-dependence of kinetic parameters
Bos taurus
4.2.1.3
additional information
-
additional information
pH-dependence of kinetic parameters
Saccharomycopsis lipolytica
4.2.1.3
0.0048
-
cis-aconitate
-
Bos taurus
4.2.1.3
0.0089
-
methyl cis-aconitate
-
Bos taurus
4.2.1.3
0.012
-
isocitrate
-
Bos taurus
4.2.1.3
0.018
-
cis-aconitate
-
Saccharomycopsis lipolytica
4.2.1.3
0.032
-
methylisocitrate
-
Bos taurus
4.2.1.3
0.045
-
isocitrate
-
Saccharomycopsis lipolytica
4.2.1.3
0.14
-
citrate
-
Bos taurus
4.2.1.3
0.158
-
methyl cis-aconitate
-
Saccharomycopsis lipolytica
4.2.1.3
0.268
-
methylisocitrate
-
Saccharomycopsis lipolytica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Bos taurus
-
-
-
4.2.1.3
Saccharomycopsis lipolytica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
-
Saccharomycopsis lipolytica
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
heart
-
Bos taurus
-
4.2.1.3
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
alpha-methyl-cis-aconitate
-
33788
Bos taurus
alpha-methylisocitrate
-
33788
Bos taurus
?
4.2.1.3
alpha-methyl-cis-aconitate
-
33788
Saccharomycopsis lipolytica
alpha-methylisocitrate
-
33788
Saccharomycopsis lipolytica
?
4.2.1.3
citrate
-
33788
Bos taurus
isocitrate
-
-
-
-
4.2.1.3
citrate
-
33788
Saccharomycopsis lipolytica
isocitrate
-
-
-
?
4.2.1.3
isocitrate
-
33788
Bos taurus
citrate
-
-
-
-
4.2.1.3
isocitrate
-
33788
Saccharomycopsis lipolytica
citrate
-
-
-
?
4.2.1.3
threo-D-alpha-methylisocitrate
-
33788
Saccharomycopsis lipolytica
?
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
pH-dependence of kinetic parameters
Bos taurus
4.2.1.3
additional information
-
additional information
pH-dependence of kinetic parameters
Saccharomycopsis lipolytica
4.2.1.3
0.0048
-
cis-aconitate
-
Bos taurus
4.2.1.3
0.0089
-
methyl cis-aconitate
-
Bos taurus
4.2.1.3
0.012
-
isocitrate
-
Bos taurus
4.2.1.3
0.018
-
cis-aconitate
-
Saccharomycopsis lipolytica
4.2.1.3
0.032
-
methylisocitrate
-
Bos taurus
4.2.1.3
0.045
-
isocitrate
-
Saccharomycopsis lipolytica
4.2.1.3
0.14
-
citrate
-
Bos taurus
4.2.1.3
0.158
-
methyl cis-aconitate
-
Saccharomycopsis lipolytica
4.2.1.3
0.268
-
methylisocitrate
-
Saccharomycopsis lipolytica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
-
Saccharomycopsis lipolytica
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
heart
-
Bos taurus
-
4.2.1.3
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
alpha-methyl-cis-aconitate
-
33788
Bos taurus
alpha-methylisocitrate
-
33788
Bos taurus
?
4.2.1.3
alpha-methyl-cis-aconitate
-
33788
Saccharomycopsis lipolytica
alpha-methylisocitrate
-
33788
Saccharomycopsis lipolytica
?
4.2.1.3
citrate
-
33788
Bos taurus
isocitrate
-
-
-
-
4.2.1.3
citrate
-
33788
Saccharomycopsis lipolytica
isocitrate
-
-
-
?
4.2.1.3
isocitrate
-
33788
Bos taurus
citrate
-
-
-
-
4.2.1.3
isocitrate
-
33788
Saccharomycopsis lipolytica
citrate
-
-
-
?
4.2.1.3
threo-D-alpha-methylisocitrate
-
33788
Saccharomycopsis lipolytica
?
-
-
-
?