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Literature summary extracted from

  • Mizobata, T.; Fujioka, T.; Yamasaki, F.; Hidaka, M.; Nagai, J.; Kawata, Y.
    Purification and characterization of a thermostable class II fumarase from Thermus thermophilus (1998), Arch. Biochem. Biophys., 355, 49-55.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.2 phosphate
-
 Thermus thermophilus

Application

EC Number Application Comment Organism
4.2.1.2 synthesis highly stable enzyme would be ideal for use in various industrial processes, especially since the specific activity is also very high Thermus thermophilus

General Stability

EC Number General Stability Organism
4.2.1.2 0.8 M guanidine hydrochloride, 50% loss of activity after 5 min at 75°C, complete inactivation at concentrations above 1.5 M Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.12
-
 (S)-malate pH 6.5, 50 mM MOPS-NaOH buffer Thermus thermophilus
4.2.1.2 0.33
-
 (S)-malate pH 7.0, 50 mM MOPS-NaOH buffer Thermus thermophilus
4.2.1.2 1
-
 (S)-malate pH 7.5, 50 mM MOPS-NaOH buffer Thermus thermophilus
4.2.1.2 2
-
 (S)-malate pH 6.5, 50 mM potassium phosphate buffer Thermus thermophilus
4.2.1.2 14
-
 (S)-malate pH 7.5, 50 mM potassium phosphate buffer Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 46000
-
 4 * 46000, SDS-PAGE Thermus thermophilus
4.2.1.2 198000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Saccharomyces cerevisiae
-
-
-
4.2.1.2 Thermus thermophilus
-
 AS-15-1, class II fumarase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 1300
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Thermus thermophilus fumarate + H2O
-
 ?
4.2.1.2 (S)-malate
-
 Saccharomyces cerevisiae fumarate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 tetramer
-
 Saccharomyces cerevisiae
4.2.1.2 tetramer 4 * 46000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 85
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.2 50 100 50°C: about 30% of maximal activity, 100°C: about 80% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 75
-
 24 h, 10% loss of activity Thermus thermophilus
4.2.1.2 90
-
 24 h, 20% loss of activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 7.5 8.5
-
 Thermus thermophilus