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Literature summary extracted from

  • Waterson, R.M.; Castellino, F.J.; Hass, G.M.; Hill, R.L.
    Purification and characterization of cortonase from Clostridium acetobutylicum (1972), J. Biol. Chem., 247, 5266-5271.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.150 lyophilization results in negligible loss of enzymatic activity Clostridium acetobutylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.17 crotonyl-CoA
-
 Clostridium acetobutylicum
4.2.1.17 additional information no inhibition by hexenoyl-CoA Clostridium acetobutylicum
4.2.1.150 crotonyl-CoA
-
 Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.17 0.03
-
 crotonyl-CoA 25°C Clostridium acetobutylicum
4.2.1.17 0.13
-
 hexenoyl-CoA 25°C Clostridium acetobutylicum
4.2.1.150 0.03
-
 crotonyl-CoA 25°C, pH 7.5 Clostridium acetobutylicum
4.2.1.150 0.13
-
 hexenoyl-CoA 25°C, pH 7.5 Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.17 40000
-
 4 * 40000, SDS-PAGE Clostridium acetobutylicum
4.2.1.17 158000
-
 equilibrium sedimentation analysis, gel filtration Clostridium acetobutylicum
4.2.1.150 43000
-
 4 * 43000, SDS-PAGE Clostridium acetobutylicum
4.2.1.150 158000
-
 sedimentation equilibrium studies Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Clostridium acetobutylicum
-
 The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-
4.2.1.150 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.17
-
 Clostridium acetobutylicum
4.2.1.150
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.17 6155
-
-
 Clostridium acetobutylicum
4.2.1.150 6155
-
 25°C, pH 7.5 Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 crotonyl-CoA + H2O the classification is ambiguous because the stereochemistry is not exactly determined Clostridium acetobutylicum ?
-
 ?
4.2.1.17 hexenoyl-CoA + H2O
-
 Clostridium acetobutylicum ?
-
 ?
4.2.1.150 a short-chain trans-2-enoyl-CoA + H2O the enzyme is specific for short chain fatty acyl-CoA substrates and is sensitive to high concentrations of crotonyl-CoA. It requires a complete coenzyme A thioester substrate for efficient catalysis Clostridium acetobutylicum short-chain (3S)-3-hydroxyacyl-CoA
-
 ?
4.2.1.150 crotonyl-CoA + H2O
-
 Clostridium acetobutylicum ?
-
 ?
4.2.1.150 hexenoyl-CoA + H2O higher analogs are not hydrated Clostridium acetobutylicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.17 tetramer 4 * 40000, SDS-PAGE Clostridium acetobutylicum
4.2.1.150 tetramer 4 * 43000, SDS-PAGE Clostridium acetobutylicum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 crotonase the classification is ambiguous because the stereochemistry of the reaction product is not exactly determined Clostridium acetobutylicum
4.2.1.150 3-hydroxybutyryl-CoA dehydratase
-
 Clostridium acetobutylicum
4.2.1.150 crotonase
-
 Clostridium acetobutylicum
4.2.1.150 crt
-
 Clostridium acetobutylicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.17 25
-
 assay at Clostridium acetobutylicum
4.2.1.150 25
-
 assay at Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.150 7.5
-
 assay at Clostridium acetobutylicum