BRENDA - Enzyme Database

Fumarase and crotonase

Hill, R.L.; Teipel, J.W.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 5, 539-571 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.2
arsenate
activates
Sus scrofa
4.2.1.2
Borate
activates
Sus scrofa
4.2.1.2
citrate
activates
Sus scrofa
4.2.1.2
phosphate
increases activity at less than 5 mM, competitive inhibition at higher concentrations
Sus scrofa
4.2.1.2
selenate
activates
Sus scrofa
4.2.1.2
SO42-
activates
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.2
4-bromocrotonate
-
Sus scrofa
4.2.1.2
ATP
-
Sus scrofa
4.2.1.2
bromofumarate
-
Sus scrofa
4.2.1.2
chlorofumarate
-
Sus scrofa
4.2.1.2
citrate
-
Sus scrofa
4.2.1.2
CTP
-
Sus scrofa
4.2.1.2
dimethylfumarate
-
Sus scrofa
4.2.1.2
GTP
-
Sus scrofa
4.2.1.2
iodoacetamide
-
Sus scrofa
4.2.1.2
iodoacetate
-
Sus scrofa
4.2.1.2
iodofumarate
-
Sus scrofa
4.2.1.2
isocitrate
L-isocitrate
Sus scrofa
4.2.1.2
L-Tartrate
-
Sus scrofa
4.2.1.2
L-threo-hydroxyaspartate
-
Sus scrofa
4.2.1.2
mesaconate
-
Sus scrofa
4.2.1.2
additional information
substrate inhibition between 0.1 M and 1.0 M
Sus scrofa
4.2.1.2
NEM
-
Sus scrofa
4.2.1.2
phosphate
increases activity at less than 5 mM, competitive inhibition at higher concentrations
Sus scrofa
4.2.1.2
trans-aconitate
-
Sus scrofa
4.2.1.2
UTP
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.2
0.005
-
fumarate
-
Sus scrofa
4.2.1.2
0.02
0.14
L-threo-chloromalate
-
Sus scrofa
4.2.1.2
0.025
-
L-malate
-
Sus scrofa
4.2.1.2
0.027
-
fluorofumarate
-
Sus scrofa
4.2.1.2
0.11
-
bromofumarate
-
Sus scrofa
4.2.1.2
0.11
-
chlorofumarate
-
Sus scrofa
4.2.1.2
0.12
-
iodofumarate
-
Sus scrofa
4.2.1.2
0.145
-
acetylene dicarboxylate
-
Sus scrofa
4.2.1.2
0.51
-
mesaconate
-
Sus scrofa
4.2.1.2
1.3
-
L-Tartrate
-
Sus scrofa
4.2.1.2
7.5
10
L-threo-hydroxyaspartate
-
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.2
48500
-
4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25
Sus scrofa
4.2.1.2
194000
-
equilibrium sedimentation
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.2
Sus scrofa
-
several isoenzymes
-
Reaction
EC Number
Reaction
Commentary
Organism
4.2.1.2
(S)-malate = fumarate + H2O
mechanism
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.2
heart
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.2
(S)-malate
-
33728
Sus scrofa
fumarate + H2O
-
-
-
r
4.2.1.2
acetylene dicarboxylate + H2O
-
33728
Sus scrofa
oxaloacetate
-
33728
Sus scrofa
?
4.2.1.2
alpha-fluorofumarate + H2O
-
33728
Sus scrofa
oxaloacetate + ?
-
-
-
?
4.2.1.2
bromofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
chlorofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
Fumarate + H2O
-
33728
Sus scrofa
L-Malate
-
33728
Sus scrofa
r
4.2.1.2
iodofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
L-tartrate
-
33728
Sus scrofa
oxaloacetate + H2O
-
33728
Sus scrofa
?
4.2.1.2
L-threo-chloro-L-malate
-
33728
Sus scrofa
chlorofumarate + H2O
-
33728
Sus scrofa
?
4.2.1.2
L-threo-hydroxyaspartate
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
mesaconate + H2O
-
33728
Sus scrofa
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.2
tetramer
4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.2
additional information
-
at low concentrations, phosphate, sulfate, borate, selenate, arsenate and citrate activate fumarase and shift somewhat the pH optimum to a more alkaline pH
Sus scrofa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.2
5
9
stable in potassium phosphate buffer, above pH 10: dissociation into an inactive form
Sus scrofa
4.2.1.2
6
10
dissociation below pH 6 and above pH 10, Tris-acetate buffer
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.2
arsenate
activates
Sus scrofa
4.2.1.2
Borate
activates
Sus scrofa
4.2.1.2
citrate
activates
Sus scrofa
4.2.1.2
phosphate
increases activity at less than 5 mM, competitive inhibition at higher concentrations
Sus scrofa
4.2.1.2
selenate
activates
Sus scrofa
4.2.1.2
SO42-
activates
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.2
4-bromocrotonate
-
Sus scrofa
4.2.1.2
ATP
-
Sus scrofa
4.2.1.2
bromofumarate
-
Sus scrofa
4.2.1.2
chlorofumarate
-
Sus scrofa
4.2.1.2
citrate
-
Sus scrofa
4.2.1.2
CTP
-
Sus scrofa
4.2.1.2
dimethylfumarate
-
Sus scrofa
4.2.1.2
GTP
-
Sus scrofa
4.2.1.2
iodoacetamide
-
Sus scrofa
4.2.1.2
iodoacetate
-
Sus scrofa
4.2.1.2
iodofumarate
-
Sus scrofa
4.2.1.2
isocitrate
L-isocitrate
Sus scrofa
4.2.1.2
L-Tartrate
-
Sus scrofa
4.2.1.2
L-threo-hydroxyaspartate
-
Sus scrofa
4.2.1.2
mesaconate
-
Sus scrofa
4.2.1.2
additional information
substrate inhibition between 0.1 M and 1.0 M
Sus scrofa
4.2.1.2
NEM
-
Sus scrofa
4.2.1.2
phosphate
increases activity at less than 5 mM, competitive inhibition at higher concentrations
Sus scrofa
4.2.1.2
trans-aconitate
-
Sus scrofa
4.2.1.2
UTP
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.2
0.005
-
fumarate
-
Sus scrofa
4.2.1.2
0.02
0.14
L-threo-chloromalate
-
Sus scrofa
4.2.1.2
0.025
-
L-malate
-
Sus scrofa
4.2.1.2
0.027
-
fluorofumarate
-
Sus scrofa
4.2.1.2
0.11
-
bromofumarate
-
Sus scrofa
4.2.1.2
0.11
-
chlorofumarate
-
Sus scrofa
4.2.1.2
0.12
-
iodofumarate
-
Sus scrofa
4.2.1.2
0.145
-
acetylene dicarboxylate
-
Sus scrofa
4.2.1.2
0.51
-
mesaconate
-
Sus scrofa
4.2.1.2
1.3
-
L-Tartrate
-
Sus scrofa
4.2.1.2
7.5
10
L-threo-hydroxyaspartate
-
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.2
48500
-
4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25
Sus scrofa
4.2.1.2
194000
-
equilibrium sedimentation
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.2
heart
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.2
(S)-malate
-
33728
Sus scrofa
fumarate + H2O
-
-
-
r
4.2.1.2
acetylene dicarboxylate + H2O
-
33728
Sus scrofa
oxaloacetate
-
33728
Sus scrofa
?
4.2.1.2
alpha-fluorofumarate + H2O
-
33728
Sus scrofa
oxaloacetate + ?
-
-
-
?
4.2.1.2
bromofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
chlorofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
Fumarate + H2O
-
33728
Sus scrofa
L-Malate
-
33728
Sus scrofa
r
4.2.1.2
iodofumarate + H2O
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
L-tartrate
-
33728
Sus scrofa
oxaloacetate + H2O
-
33728
Sus scrofa
?
4.2.1.2
L-threo-chloro-L-malate
-
33728
Sus scrofa
chlorofumarate + H2O
-
33728
Sus scrofa
?
4.2.1.2
L-threo-hydroxyaspartate
-
33728
Sus scrofa
?
-
-
-
?
4.2.1.2
mesaconate + H2O
-
33728
Sus scrofa
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.2
tetramer
4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.2
additional information
-
at low concentrations, phosphate, sulfate, borate, selenate, arsenate and citrate activate fumarase and shift somewhat the pH optimum to a more alkaline pH
Sus scrofa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.2.1.2
5
9
stable in potassium phosphate buffer, above pH 10: dissociation into an inactive form
Sus scrofa
4.2.1.2
6
10
dissociation below pH 6 and above pH 10, Tris-acetate buffer
Sus scrofa