BRENDA - Enzyme Database

Purification and properties of pig liver and muscle enolases

Farrar, W.W.; Deal, W.C.; J. Protein Chem. 14, 487-497 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Li+
liver enzyme is severely inhibited, muscle enzyme is moderately inhibited
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.09
-
2-phosphoglycerate
pH 6.8, muscle enolase; pH 7.4, liver enolase and muscle enolase
Sus scrofa
4.2.1.11
0.17
-
2-phosphoglycerate
pH 6.8, liver enolase
Sus scrofa
4.2.1.11
0.17
-
phosphoenolpyruvate
pH 7.4, muscle enolase
Sus scrofa
4.2.1.11
0.19
-
phosphoenolpyruvate
pH 6.8, liver enolase
Sus scrofa
4.2.1.11
0.25
-
phosphoenolpyruvate
pH 7.4, liver enolase
Sus scrofa
4.2.1.11
0.33
-
phosphoenolpyruvate
pH 6.8, muscle enolase
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
at pH 6.8, Km for liver enzyme: 0.67 mM, Km for muscle enzyme: 1.33 mM at pH 7.4, Km for liver enolase: 0.42 mM, Km for muscle enolase: 0.25 mM
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
44000
-
2 * 44000, muscle enzyme, SDS-PAGE
Sus scrofa
4.2.1.11
46000
-
2 * 46000, liver enzyme, SDS-PAGE
Sus scrofa
4.2.1.11
84970
-
muscle enolase, calculation from amino acid composition
Sus scrofa
4.2.1.11
85000
-
muscle enzyme, equilibrium sedimentation
Sus scrofa
4.2.1.11
89020
-
liver enolase, calculation from amino acid composition
Sus scrofa
4.2.1.11
92000
-
liver enzyme, equilibrium sedimentation
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
64.6
-
liver enolase
Sus scrofa
4.2.1.11
91
-
muscle enolase
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33639
Sus scrofa
phosphoenolpyruvate
-
33639
Sus scrofa
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 44000, muscle enzyme, SDS-PAGE; 2 * 46000, liver enzyme, SDS-PAGE
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
-
muscle enolase, formation of 2-phosphoglycerate
Sus scrofa
4.2.1.11
6.8
-
muscle enolase, formation of phosphoenolpyruvate
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Li+
liver enzyme is severely inhibited, muscle enzyme is moderately inhibited
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.09
-
2-phosphoglycerate
pH 6.8, muscle enolase; pH 7.4, liver enolase and muscle enolase
Sus scrofa
4.2.1.11
0.17
-
2-phosphoglycerate
pH 6.8, liver enolase
Sus scrofa
4.2.1.11
0.17
-
phosphoenolpyruvate
pH 7.4, muscle enolase
Sus scrofa
4.2.1.11
0.19
-
phosphoenolpyruvate
pH 6.8, liver enolase
Sus scrofa
4.2.1.11
0.25
-
phosphoenolpyruvate
pH 7.4, liver enolase
Sus scrofa
4.2.1.11
0.33
-
phosphoenolpyruvate
pH 6.8, muscle enolase
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
at pH 6.8, Km for liver enzyme: 0.67 mM, Km for muscle enzyme: 1.33 mM at pH 7.4, Km for liver enolase: 0.42 mM, Km for muscle enolase: 0.25 mM
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
44000
-
2 * 44000, muscle enzyme, SDS-PAGE
Sus scrofa
4.2.1.11
46000
-
2 * 46000, liver enzyme, SDS-PAGE
Sus scrofa
4.2.1.11
84970
-
muscle enolase, calculation from amino acid composition
Sus scrofa
4.2.1.11
85000
-
muscle enzyme, equilibrium sedimentation
Sus scrofa
4.2.1.11
89020
-
liver enolase, calculation from amino acid composition
Sus scrofa
4.2.1.11
92000
-
liver enzyme, equilibrium sedimentation
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
64.6
-
liver enolase
Sus scrofa
4.2.1.11
91
-
muscle enolase
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33639
Sus scrofa
phosphoenolpyruvate
-
33639
Sus scrofa
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 44000, muscle enzyme, SDS-PAGE; 2 * 46000, liver enzyme, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
-
muscle enolase, formation of 2-phosphoglycerate
Sus scrofa
4.2.1.11
6.8
-
muscle enolase, formation of phosphoenolpyruvate
Sus scrofa