Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gorisch, S.W.; Barrows, V.; Halbert, J.; Farrar, W.W.
    Purification and properties of gammagamma-enolase from pig brain (1999), J. Protein Chem., 18, 103-115.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.11
-
 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 Li+ noncompetitive with either 2-phosphoglycerate or Mg2+ Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.05
-
 2-phospho-D-glycerate
-
 Sus scrofa
4.2.1.11 0.25
-
 phosphoenolpyruvate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ Km: 0.5 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 45000
-
 2 * 45000, SDS-PAGE Sus scrofa
4.2.1.11 85000
-
 sucrose density gradient ultracentrifugation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Sus scrofa
-
 3 enolase isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 gamma,gamma-enolase Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 brain
-
 Sus scrofa
-
4.2.1.11 muscle
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 82.2
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 phosphoenolpyruvate
-
 Sus scrofa 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 45000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 7.2
-
-
 Sus scrofa
4.2.1.11 7.4
-
 liver enzyme, both directions Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.11 6.8 8 about 55% of maximal activity at pH 6.8 and at pH 8.0 Sus scrofa