BRENDA - Enzyme Database

Purification and properties of gammagamma-enolase from pig brain

Gorisch, S.W.; Barrows, V.; Halbert, J.; Farrar, W.W.; J. Protein Chem. 18, 103-115 (1999)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
-
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Li+
noncompetitive with either 2-phosphoglycerate or Mg2+
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.05
-
2-phosphoglycerate
-
Sus scrofa
4.2.1.11
0.25
-
phosphoenolpyruvate
-
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km: 0.5 mM
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
2 * 45000, SDS-PAGE
Sus scrofa
4.2.1.11
85000
-
sucrose density gradient ultracentrifugation
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Sus scrofa
-
3 enolase isoforms
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
gamma,gamma-enolase
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
-
Sus scrofa
-
4.2.1.11
muscle
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
82.2
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33638
Sus scrofa
phosphoenolpyruvate
-
33638
Sus scrofa
r
4.2.1.11
phosphoenolpyruvate
-
33638
Sus scrofa
2-phospho-D-glycerate
-
33638
Sus scrofa
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 45000, SDS-PAGE
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.2
-
-
Sus scrofa
4.2.1.11
7.4
-
liver enzyme, both directions
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6.8
8
about 55% of maximal activity at pH 6.8 and at pH 8.0
Sus scrofa
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
-
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Li+
noncompetitive with either 2-phosphoglycerate or Mg2+
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.05
-
2-phosphoglycerate
-
Sus scrofa
4.2.1.11
0.25
-
phosphoenolpyruvate
-
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km: 0.5 mM
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
2 * 45000, SDS-PAGE
Sus scrofa
4.2.1.11
85000
-
sucrose density gradient ultracentrifugation
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
gamma,gamma-enolase
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
-
Sus scrofa
-
4.2.1.11
muscle
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
82.2
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33638
Sus scrofa
phosphoenolpyruvate
-
33638
Sus scrofa
r
4.2.1.11
phosphoenolpyruvate
-
33638
Sus scrofa
2-phospho-D-glycerate
-
33638
Sus scrofa
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 45000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.2
-
-
Sus scrofa
4.2.1.11
7.4
-
liver enzyme, both directions
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6.8
8
about 55% of maximal activity at pH 6.8 and at pH 8.0
Sus scrofa