BRENDA - Enzyme Database

Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: purification, characterization, and image processing

Schurig, H.; Rutkat, K.; Rachel, R.; Jaenicke, R.; Protein Sci. 4, 228-236 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
in absence of phosphate noncompetitive inhibition up to 10 mM F-, competitive inhibition in presence of 0.5 mM phosphate
Thermotoga maritima
4.2.1.11
phosphate
-
Thermotoga maritima
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
temperature-dependence of Km-values
Thermotoga maritima
4.2.1.11
0.07
-
2-phosphoglycerate
at 75C
Thermotoga maritima
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.03 mM
Thermotoga maritima
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
8 * 48000, a tetramer of dimers, SDS-PAGE
Thermotoga maritima
4.2.1.11
345000
-
gel filtration, high-speed equilibrium sedimentation
Thermotoga maritima
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Thermotoga maritima
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Thermotoga maritima
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33637
Thermotoga maritima
phosphoenolpyruvate
-
33637
Thermotoga maritima
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 48000, a tetramer of dimers, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.11
90
-
-
Thermotoga maritima
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
at 70C
Thermotoga maritima
4.2.1.11
8
-
20C
Thermotoga maritima
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
in absence of phosphate noncompetitive inhibition up to 10 mM F-, competitive inhibition in presence of 0.5 mM phosphate
Thermotoga maritima
4.2.1.11
phosphate
-
Thermotoga maritima
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
temperature-dependence of Km-values
Thermotoga maritima
4.2.1.11
0.07
-
2-phosphoglycerate
at 75C
Thermotoga maritima
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.03 mM
Thermotoga maritima
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
8 * 48000, a tetramer of dimers, SDS-PAGE
Thermotoga maritima
4.2.1.11
345000
-
gel filtration, high-speed equilibrium sedimentation
Thermotoga maritima
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Thermotoga maritima
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33637
Thermotoga maritima
phosphoenolpyruvate
-
33637
Thermotoga maritima
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 48000, a tetramer of dimers, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.11
90
-
-
Thermotoga maritima
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
at 70C
Thermotoga maritima
4.2.1.11
8
-
20C
Thermotoga maritima