BRENDA - Enzyme Database

Biochemical and immunochemical properties of modified human neuron-specific enolase

Kaneta, M.; Aoki, T.; Onagi, H.; Morikawa, J.; Watabe, H.; Biol. Pharm. Bull. 20, 556-559 (1997)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli; neuron specific enolase and modified neuron-specific enolases: Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
recombinant neuron-specific enolase (R-NSE) has markedly decreased binding affinity to anti-neuron-specific enolase antibodies. Reactivity of modified neuron-specific enolases (Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase. Y-NSE.H6 with six His residues further added at the C-terminal of recombinant neuron-specific enolase) to the antibody is almost equivalent to that of human brain gamma,gamma-enolase
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.22
-
2-phosphoglycerate
Y-NSE.H6
Homo sapiens
4.2.1.11
0.24
-
2-phosphoglycerate
R-NSE
Homo sapiens
4.2.1.11
0.25
-
2-phosphoglycerate
Y-NSE
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE
Homo sapiens
4.2.1.11
47000
-
x * 47000, Y-NSE.H6, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Homo sapiens
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33634
Homo sapiens
phosphoenolpyruvate
-
33634
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE; x * 47000, Y-NSE.H6, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.4
7.6
R-NSE, Y-NSE, Y-NSE.H6
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli; neuron specific enolase and modified neuron-specific enolases: Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
recombinant neuron-specific enolase (R-NSE) has markedly decreased binding affinity to anti-neuron-specific enolase antibodies. Reactivity of modified neuron-specific enolases (Y-NSE with one Tyr residue added at the N-terminal of the recombinant neuron-specific enolase. Y-NSE.H6 with six His residues further added at the C-terminal of recombinant neuron-specific enolase) to the antibody is almost equivalent to that of human brain gamma,gamma-enolase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.22
-
2-phosphoglycerate
Y-NSE.H6
Homo sapiens
4.2.1.11
0.24
-
2-phosphoglycerate
R-NSE
Homo sapiens
4.2.1.11
0.25
-
2-phosphoglycerate
Y-NSE
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE
Homo sapiens
4.2.1.11
47000
-
x * 47000, Y-NSE.H6, SDS-PAGE
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33634
Homo sapiens
phosphoenolpyruvate
-
33634
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46000, enzyme R-NSE, enzyme Y-NSE, SDS-PAGE; x * 47000, Y-NSE.H6, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.4
7.6
R-NSE, Y-NSE, Y-NSE.H6
Homo sapiens