BRENDA - Enzyme Database

Purification, characterization and inhibition by fluoride of enolase from Streptococcus mutans DSM 320523

Kaufmann, M.; Batholmes, P.; Caries Res. 26, 110-116 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
noncompetitive inhibition below 10 mM, competitive above 10 mM
Streptococcus mutans
4.2.1.11
additional information
inhibition of enolase by fluoride in combination with phosphate can influence glycolysis and so reduce acid production of even growth rate, thereby leading to potential anticariogenic effects
Streptococcus mutans
4.2.1.11
Na2PO3F
-
Streptococcus mutans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.44
-
2-phosphoglycerate
in presence of 1 mM Mg2+
Streptococcus mutans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
8 * 45000, SDS-PAGE
Streptococcus mutans
4.2.1.11
350000
-
equilibrium sedimentation
Streptococcus mutans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus mutans
-
DSM 320523
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Streptococcus mutans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33632
Streptococcus mutans
phosphoenolpyruvate
-
33632
Streptococcus mutans
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 45000, SDS-PAGE
Streptococcus mutans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
noncompetitive inhibition below 10 mM, competitive above 10 mM
Streptococcus mutans
4.2.1.11
additional information
inhibition of enolase by fluoride in combination with phosphate can influence glycolysis and so reduce acid production of even growth rate, thereby leading to potential anticariogenic effects
Streptococcus mutans
4.2.1.11
Na2PO3F
-
Streptococcus mutans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.44
-
2-phosphoglycerate
in presence of 1 mM Mg2+
Streptococcus mutans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
45000
-
8 * 45000, SDS-PAGE
Streptococcus mutans
4.2.1.11
350000
-
equilibrium sedimentation
Streptococcus mutans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Streptococcus mutans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33632
Streptococcus mutans
phosphoenolpyruvate
-
33632
Streptococcus mutans
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 45000, SDS-PAGE
Streptococcus mutans