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Literature summary extracted from
- Isolation, characterization, and inhibition kinetics of enolase from Streptococcus rattus FA-1 (1990), Infect. Immun., 58, 1043-1047.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | phosphate | slight stimulation without F- | Streptococcus ratti |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | 2-phosphoglycerate | competitive | Streptococcus ratti | |
| 4.2.1.11 | F- | - |
Streptococcus ratti | |
| 4.2.1.11 | monofluorophosphate | - |
Streptococcus ratti | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | 4.35 | - |
2-phospho-D-glycerate | - |
Streptococcus ratti | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 22000 | - |
monomeric enzyme form, gel filtration, nondenaturing PAGE | Streptococcus ratti |
| 4.2.1.11 | 49000 | - |
dimeric enzyme form, gel filtration, native PAGE | Streptococcus ratti |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Streptococcus ratti | - |
FA-1 | - |
| 4.2.1.11 | Streptococcus ratti FA-1 | - |
FA-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | - |
Streptococcus ratti |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 918 | - |
monomeric enzyme form | Streptococcus ratti |
| 4.2.1.11 | 1118 | - |
dimeric enzyme form | Streptococcus ratti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Streptococcus ratti | phosphoenolpyruvate | - |
? | |
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Streptococcus ratti FA-1 | phosphoenolpyruvate | - |
? | |
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Streptococcus ratti | phosphoenolpyruvate + H2O | - |
? | |
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Streptococcus ratti FA-1 | phosphoenolpyruvate + H2O | - |
? | |
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