BRENDA - Enzyme Database

Isolation, characterization, and inhibition kinetics of enolase from Streptococcus rattus FA-1

Huether, F.J.; Psarros, N.; Duschner, H.; Infect. Immun. 58, 1043-1047 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.11
phosphate
slight stimulation without F-
Streptococcus ratti
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
competitive
Streptococcus ratti
4.2.1.11
F-
-
Streptococcus ratti
4.2.1.11
monofluorophosphate
-
Streptococcus ratti
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
4.35
-
2-phosphoglycerate
-
Streptococcus ratti
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
22000
-
monomeric enzyme form, gel filtration, nondenaturing PAGE
Streptococcus ratti
4.2.1.11
49000
-
dimeric enzyme form, gel filtration, native PAGE
Streptococcus ratti
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus ratti
-
FA-1
-
4.2.1.11
Streptococcus ratti FA-1
-
FA-1
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Streptococcus ratti
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
918
-
monomeric enzyme form
Streptococcus ratti
4.2.1.11
1118
-
dimeric enzyme form
Streptococcus ratti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33630
Streptococcus ratti
phosphoenolpyruvate
-
33630
Streptococcus ratti
?
4.2.1.11
2-phospho-D-glycerate
-
33630
Streptococcus ratti FA-1
phosphoenolpyruvate
-
33630
Streptococcus ratti FA-1
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.11
phosphate
slight stimulation without F-
Streptococcus ratti
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
competitive
Streptococcus ratti
4.2.1.11
F-
-
Streptococcus ratti
4.2.1.11
monofluorophosphate
-
Streptococcus ratti
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
4.35
-
2-phosphoglycerate
-
Streptococcus ratti
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
22000
-
monomeric enzyme form, gel filtration, nondenaturing PAGE
Streptococcus ratti
4.2.1.11
49000
-
dimeric enzyme form, gel filtration, native PAGE
Streptococcus ratti
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Streptococcus ratti
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
918
-
monomeric enzyme form
Streptococcus ratti
4.2.1.11
1118
-
dimeric enzyme form
Streptococcus ratti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33630
Streptococcus ratti
phosphoenolpyruvate
-
33630
Streptococcus ratti
?
4.2.1.11
2-phospho-D-glycerate
-
33630
Streptococcus ratti FA-1
phosphoenolpyruvate
-
33630
Streptococcus ratti FA-1
?