BRENDA - Enzyme Database

Characterization of enolase from Clostridium difficile

Green, G.A.; Girardot, R.; Baldacini, O.; Ledig, M.; Monteil, H.; Curr. Microbiol. 26, 53-56 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
in presence of phosphate, competitive
Clostridioides difficile
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
optimal concentration: 1 mM
Clostridioides difficile
4.2.1.11
Zn2+
optimal concentration: 1 mM
Clostridioides difficile
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, SDS-PAGE
Clostridioides difficile
4.2.1.11
300000
-
non-denaturing PAGE
Clostridioides difficile
4.2.1.11
338000
-
gel filtration
Clostridioides difficile
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Clostridioides difficile
-
-
-
4.2.1.11
Paeniclostridium sordellii
-
-
-
4.2.1.11
Paraclostridium bifermentans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Clostridioides difficile
4.2.1.11
partial
Paeniclostridium sordellii
4.2.1.11
partial
Paraclostridium bifermentans
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
-20C, 10 mM Tris-HCL, pH 7.5, stable
Clostridioides difficile
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33627
Clostridioides difficile
phosphoenolpyruvate
-
33627
Clostridioides difficile
?
4.2.1.11
2-phospho-D-glycerate
-
33627
Paeniclostridium sordellii
phosphoenolpyruvate
-
33627
Paeniclostridium sordellii
?
4.2.1.11
2-phospho-D-glycerate
-
33627
Paraclostridium bifermentans
phosphoenolpyruvate
-
33627
Paraclostridium bifermentans
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, SDS-PAGE
Clostridioides difficile
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.11
25
50
-
Clostridioides difficile
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.2.1.11
25
80
25-50C: maximal activity, 60C, 89% of maximal activity, 70C: 57% of maximal activity; 80C: 27% of maximal activity
Clostridioides difficile
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.6
-
-
Clostridioides difficile
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
in presence of phosphate, competitive
Clostridioides difficile
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
optimal concentration: 1 mM
Clostridioides difficile
4.2.1.11
Zn2+
optimal concentration: 1 mM
Clostridioides difficile
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, SDS-PAGE
Clostridioides difficile
4.2.1.11
300000
-
non-denaturing PAGE
Clostridioides difficile
4.2.1.11
338000
-
gel filtration
Clostridioides difficile
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Clostridioides difficile
4.2.1.11
partial
Paeniclostridium sordellii
4.2.1.11
partial
Paraclostridium bifermentans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
-20C, 10 mM Tris-HCL, pH 7.5, stable
Clostridioides difficile
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33627
Clostridioides difficile
phosphoenolpyruvate
-
33627
Clostridioides difficile
?
4.2.1.11
2-phospho-D-glycerate
-
33627
Paeniclostridium sordellii
phosphoenolpyruvate
-
33627
Paeniclostridium sordellii
?
4.2.1.11
2-phospho-D-glycerate
-
33627
Paraclostridium bifermentans
phosphoenolpyruvate
-
33627
Paraclostridium bifermentans
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, SDS-PAGE
Clostridioides difficile
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.11
25
50
-
Clostridioides difficile
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.2.1.11
25
80
25-50C: maximal activity, 60C, 89% of maximal activity, 70C: 57% of maximal activity; 80C: 27% of maximal activity
Clostridioides difficile
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.6
-
-
Clostridioides difficile