BRENDA - Enzyme Database

Biochemical characterization of the mouse muscle-specific enolase: developmental changes in electrophoretic variants and selective binding to other proteins

Merkulova, T.; Lucas, M.; Jabet, C.; Lamande, N.; Rouzeau, J.D.; Gros, F.; Lazar, M.; Keller, A.; Biochem. J. 323, 791-800 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.078
-
2-phosphoglycerate
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
beta,beta-enolase binds with high affinity the adjacent enzymes in the glycolytic pathway (pyruvate kinase and phosphoglycerate mutase), beta,beta-enolase binds with high affinity sarcomeric troponin but not actin and tropomyosin
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
beta,beta-enolase
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
the embryonic alpha,alpha isoform remains distributed in many adult cell types, wheras a transition towards beta,beta-isoform and gamma,gamma-isoform occurs in striated muscle cells and neurons respectively
Mus musculus
-
4.2.1.11
muscle
hindlimb muscle
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Mus musculus
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
-74°C, pH 7.0, stable for up to 10 months, provided that multiple freezing and thawing steps are avoided
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33625
Mus musculus
phosphoenolpyruvate
-
33625
Mus musculus
?
4.2.1.11
2-phospho-D-glycerate
beta,beta-enolase binds with high affinity the adjacent enzymes in the glycolytic pathway (pyruvate kinase and phosphoglycerate mutase), beta,beta-enolase binds with high affinity sarcomeric troponin but not actin and tropomyosin
33625
Mus musculus
?
-
-
-
-
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.078
-
2-phosphoglycerate
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
beta,beta-enolase binds with high affinity the adjacent enzymes in the glycolytic pathway (pyruvate kinase and phosphoglycerate mutase), beta,beta-enolase binds with high affinity sarcomeric troponin but not actin and tropomyosin
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
beta,beta-enolase
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
the embryonic alpha,alpha isoform remains distributed in many adult cell types, wheras a transition towards beta,beta-isoform and gamma,gamma-isoform occurs in striated muscle cells and neurons respectively
Mus musculus
-
4.2.1.11
muscle
hindlimb muscle
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Mus musculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
-74°C, pH 7.0, stable for up to 10 months, provided that multiple freezing and thawing steps are avoided
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33625
Mus musculus
phosphoenolpyruvate
-
33625
Mus musculus
?
4.2.1.11
2-phospho-D-glycerate
beta,beta-enolase binds with high affinity the adjacent enzymes in the glycolytic pathway (pyruvate kinase and phosphoglycerate mutase), beta,beta-enolase binds with high affinity sarcomeric troponin but not actin and tropomyosin
33625
Mus musculus
?
-
-
-
-