BRENDA - Enzyme Database

Enolase isozymes from Ricinus communis: partial purification and characterization of the isozymes

Miernyk, J.A.; Dennis, D.T.; Arch. Biochem. Biophys. 233, 643-651 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
competitive
Ricinus communis
4.2.1.11
F-
-
Ricinus communis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.025
-
2-phosphoglycerate
plastidic isoenzyme
Ricinus communis
4.2.1.11
0.061
-
2-phosphoglycerate
cytosolic isoenzyme
Ricinus communis
4.2.1.11
2.6
-
phosphoenolpyruvate
plastidic isoenzyme
Ricinus communis
4.2.1.11
7.2
-
phosphoenolpyruvate
cytosolic isoenzyme
Ricinus communis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Ricinus communis
5829
-
4.2.1.11
plastid
-
Ricinus communis
9536
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Co2+
less than 40% of the activation with Mg2+
Ricinus communis
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective
Ricinus communis
4.2.1.11
Mn2+
10% of the activation with Mg2+; absolute requirement for divalent metal ions; Km for the cytoplasmic isoenzyme: 1.2 mM; Km for the plastidic isoenzyme: 1.4 mM
Ricinus communis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Ricinus communis
-
L. Cv. Baker 296
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
partial
Ricinus communis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
endosperm
developing
Ricinus communis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
197.4
-
plastidic isoenzyme
Ricinus communis
4.2.1.11
200
-
-
Ricinus communis
4.2.1.11
252.4
-
cytosolic isoenzyme
Ricinus communis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33620
Ricinus communis
phosphoenolpyruvate
-
33620
Ricinus communis
r
4.2.1.11
phosphoenolpyruvate
-
33620
Ricinus communis
2-phospho-D-glycerate
-
33620
Ricinus communis
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6
-
formation of 2-phosphoglycerate, plastidic isoenzyme
Ricinus communis
4.2.1.11
7.5
-
formation of phosphoenolpyruvate
Ricinus communis
4.2.1.11
7.8
-
formation of 2-phosphoglycerate, cytosolic isoenzyme
Ricinus communis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
competitive
Ricinus communis
4.2.1.11
F-
-
Ricinus communis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.025
-
2-phosphoglycerate
plastidic isoenzyme
Ricinus communis
4.2.1.11
0.061
-
2-phosphoglycerate
cytosolic isoenzyme
Ricinus communis
4.2.1.11
2.6
-
phosphoenolpyruvate
plastidic isoenzyme
Ricinus communis
4.2.1.11
7.2
-
phosphoenolpyruvate
cytosolic isoenzyme
Ricinus communis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Ricinus communis
5829
-
4.2.1.11
plastid
-
Ricinus communis
9536
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Co2+
less than 40% of the activation with Mg2+
Ricinus communis
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective
Ricinus communis
4.2.1.11
Mn2+
10% of the activation with Mg2+; absolute requirement for divalent metal ions; Km for the cytoplasmic isoenzyme: 1.2 mM; Km for the plastidic isoenzyme: 1.4 mM
Ricinus communis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
partial
Ricinus communis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
endosperm
developing
Ricinus communis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
197.4
-
plastidic isoenzyme
Ricinus communis
4.2.1.11
200
-
-
Ricinus communis
4.2.1.11
252.4
-
cytosolic isoenzyme
Ricinus communis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33620
Ricinus communis
phosphoenolpyruvate
-
33620
Ricinus communis
r
4.2.1.11
phosphoenolpyruvate
-
33620
Ricinus communis
2-phospho-D-glycerate
-
33620
Ricinus communis
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6
-
formation of 2-phosphoglycerate, plastidic isoenzyme
Ricinus communis
4.2.1.11
7.5
-
formation of phosphoenolpyruvate
Ricinus communis
4.2.1.11
7.8
-
formation of 2-phosphoglycerate, cytosolic isoenzyme
Ricinus communis