BRENDA - Enzyme Database

Purification and comparative characterization of an enolase from spinach

Sinha, S.; Brewer, J.M.; Plant Physiol. 74, 834-840 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.11
additional information
-
Solanum tuberosum
4.2.1.11
additional information
contains 23 half-cystines per mol of subunit
Spinacia oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
-
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.045
-
2-phosphoglycerate
-
Spinacia oleracea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Cd2+
14% of the activation with Mg2+
Spinacia oleracea
4.2.1.11
Cu2+
20% of the activiation with Mg2+
Spinacia oleracea
4.2.1.11
Mg2+
activation by several divalent cation, Mg2+ is most effective
Spinacia oleracea
4.2.1.11
Mn2+
19% of the activation with Mg2+
Spinacia oleracea
4.2.1.11
Zn2+
12% of the activation with Mg2+
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
2 * 50000, SDS-PAGE
Spinacia oleracea
4.2.1.11
93000
-
gel filtration
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Solanum tuberosum
-
-
-
4.2.1.11
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Spinacia oleracea
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33619
Spinacia oleracea
phosphoenolpyruvate
-
33619
Spinacia oleracea
?
4.2.1.11
2-phospho-D-glycerate
-
33619
Solanum tuberosum
phosphoenolpyruvate
-
33619
Solanum tuberosum
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 50000, SDS-PAGE
Spinacia oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.8
8
-
Spinacia oleracea
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.11
additional information
-
Solanum tuberosum
4.2.1.11
additional information
contains 23 half-cystines per mol of subunit
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
-
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.045
-
2-phosphoglycerate
-
Spinacia oleracea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Cd2+
14% of the activation with Mg2+
Spinacia oleracea
4.2.1.11
Cu2+
20% of the activiation with Mg2+
Spinacia oleracea
4.2.1.11
Mg2+
activation by several divalent cation, Mg2+ is most effective
Spinacia oleracea
4.2.1.11
Mn2+
19% of the activation with Mg2+
Spinacia oleracea
4.2.1.11
Zn2+
12% of the activation with Mg2+
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
2 * 50000, SDS-PAGE
Spinacia oleracea
4.2.1.11
93000
-
gel filtration
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Spinacia oleracea
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33619
Spinacia oleracea
phosphoenolpyruvate
-
33619
Spinacia oleracea
?
4.2.1.11
2-phospho-D-glycerate
-
33619
Solanum tuberosum
phosphoenolpyruvate
-
33619
Solanum tuberosum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 50000, SDS-PAGE
Spinacia oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.8
8
-
Spinacia oleracea