BRENDA - Enzyme Database

Purification of three distinct enolase isoenzymes from yeast

Entian, K.D.; Meurer, B.; Mecke, D.; Anal. Biochem. 132, 225-228 (1983)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
77000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
-
strain cat 1.S3-14A
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
135
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
4°C, 15% ethylene glycol, stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33617
Saccharomyces cerevisiae
phosphoenolpyruvate
-
33617
Saccharomyces cerevisiae
?
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
77000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
135
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
4°C, 15% ethylene glycol, stable for at least 3 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33617
Saccharomyces cerevisiae
phosphoenolpyruvate
-
33617
Saccharomyces cerevisiae
?