BRENDA - Enzyme Database

Purification, characterization, and distribution of enolase isozymes in chicken

Tanaka, M.; Sugisaki, K.; Nakashima, K.; J. Biochem. 98, 1527-1534 (1985)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.029
-
2-phosphoglycerate
brain enolase I, brain enolase II, brain enolase III
Gallus gallus
4.2.1.11
0.038
-
2-phosphoglycerate
muscle enolase
Gallus gallus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
51000
-
alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE; alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE
Gallus gallus
4.2.1.11
51500
-
beta,beta, 2 * 51500, muscle enolase, SDS-PAGE
Gallus gallus
4.2.1.11
52000
-
alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE; gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE
Gallus gallus
4.2.1.11
100000
-
gel filtration
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
enolase I (alpha,alpha-enolase), enolase II (alpha,gamma-enolase), enolase III (gamma,gamma-enolase); the brain alpha,gamma and gamma,gamma forms are not detectable in the early embryonic stage and increase gradually during the development of the brain, whereas the alpha,alpha form exists at an almost constant level during development
Gallus gallus
-
4.2.1.11
skeletal muscle
beta,beta-enolase, complete switching from alpha,alpha enolase to beta,beta enolase during the period around hatching; contains a single enzyme form
Gallus gallus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
79.8
-
brain enolase III
Gallus gallus
4.2.1.11
82.8
-
brain enolase I
Gallus gallus
4.2.1.11
92.3
-
muscle enolase
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33614
Gallus gallus
phosphoenolpyruvate
-
33614
Gallus gallus
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE; alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE; beta,beta, 2 * 51500, muscle enolase, SDS-PAGE; gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE
Gallus gallus
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.2.1.11
55
-
60 min, in presence of Mg2+, enolase II retains more than 40% of its activity, brain enolase I and II lose 80% of their activities. In absence of Mg2+ all enolases quickly lose activity
Gallus gallus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
brain enolase I, brain enolase III; muscle enolase
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.029
-
2-phosphoglycerate
brain enolase I, brain enolase II, brain enolase III
Gallus gallus
4.2.1.11
0.038
-
2-phosphoglycerate
muscle enolase
Gallus gallus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
51000
-
alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE; alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE
Gallus gallus
4.2.1.11
51500
-
beta,beta, 2 * 51500, muscle enolase, SDS-PAGE
Gallus gallus
4.2.1.11
52000
-
alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE; gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE
Gallus gallus
4.2.1.11
100000
-
gel filtration
Gallus gallus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
enolase I (alpha,alpha-enolase), enolase II (alpha,gamma-enolase), enolase III (gamma,gamma-enolase); the brain alpha,gamma and gamma,gamma forms are not detectable in the early embryonic stage and increase gradually during the development of the brain, whereas the alpha,alpha form exists at an almost constant level during development
Gallus gallus
-
4.2.1.11
skeletal muscle
beta,beta-enolase, complete switching from alpha,alpha enolase to beta,beta enolase during the period around hatching; contains a single enzyme form
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
79.8
-
brain enolase III
Gallus gallus
4.2.1.11
82.8
-
brain enolase I
Gallus gallus
4.2.1.11
92.3
-
muscle enolase
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33614
Gallus gallus
phosphoenolpyruvate
-
33614
Gallus gallus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE; alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE; beta,beta, 2 * 51500, muscle enolase, SDS-PAGE; gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE
Gallus gallus
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.2.1.11
55
-
60 min, in presence of Mg2+, enolase II retains more than 40% of its activity, brain enolase I and II lose 80% of their activities. In absence of Mg2+ all enolases quickly lose activity
Gallus gallus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
brain enolase I, brain enolase III; muscle enolase
Gallus gallus