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Literature summary extracted from

  • Suzuki, F.; Umeda, Y.; Kato, K.
    Rat brain enolase isozymes. Purification of three forms of enolase (1980), J. Biochem., 87, 1587-1594.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.036
-
 2-phospho-D-glycerate enolase III Rattus norvegicus
4.2.1.11 0.045
-
 2-phospho-D-glycerate enolase II Rattus norvegicus
4.2.1.11 0.057
-
 2-phospho-D-glycerate enolase I Rattus norvegicus
4.2.1.11 0.14
-
 phosphoenolpyruvate enolase III Rattus norvegicus
4.2.1.11 0.15
-
 phosphoenolpyruvate enolase I Rattus norvegicus
4.2.1.11 0.19
-
 phosphoenolpyruvate enolase II Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 46000
-
 1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus
4.2.1.11 46000
-
 2 * 46000, enolase III, SDS-PAGE Rattus norvegicus
4.2.1.11 49000
-
 2 * 49000, enolase I, SDS-PAGE Rattus norvegicus
4.2.1.11 49000
-
 1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Rattus norvegicus
-
 3 forms of the enzyme: enolase I, enolase II and enolase III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 enolase 1, enolase II, enolase III Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 brain enolase I (alpha,alpha-enolase), enolase II (alpha,gamma-enolase), enolase III (gamma,gamma-enolase) Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 51
-
 enolase III Rattus norvegicus
4.2.1.11 68
-
 enolase II Rattus norvegicus
4.2.1.11 87
-
 enolase I Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Rattus norvegicus phosphoenolpyruvate
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Rattus norvegicus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 phosphoenolpyruvate
-
 Rattus norvegicus 2-phospho-D-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 49000, enolase I, SDS-PAGE Rattus norvegicus
4.2.1.11 dimer 1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus
4.2.1.11 dimer 2 * 46000, enolase III, SDS-PAGE Rattus norvegicus