BRENDA - Enzyme Database

Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties

Singh, R.P.; Setlow, P.; J. Bacteriol. 134, 353-355 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
EDTA
-
Bacillus megaterium
4.2.1.11
F-
-
Bacillus megaterium
4.2.1.11
additional information
no inhibition by NEM and iodoacetate
Bacillus megaterium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.71
-
2-phosphoglycerate
-
Bacillus megaterium
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km: 0.47 mM; required
Bacillus megaterium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
42000
-
8 * 42000, SDS-PAGE
Bacillus megaterium
4.2.1.11
335000
-
gel filtration
Bacillus megaterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Bacillus megaterium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Bacillus megaterium
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
spore
germinated
Bacillus megaterium
-
4.2.1.11
vegetative
-
Bacillus megaterium
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Bacillus megaterium
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
in the frozen state, stable for 6 months
Bacillus megaterium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33609
Bacillus megaterium
phosphoenolpyruvate
-
33609
Bacillus megaterium
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 42000, SDS-PAGE
Bacillus megaterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8
-
-
Bacillus megaterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
EDTA
-
Bacillus megaterium
4.2.1.11
F-
-
Bacillus megaterium
4.2.1.11
additional information
no inhibition by NEM and iodoacetate
Bacillus megaterium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.71
-
2-phosphoglycerate
-
Bacillus megaterium
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km: 0.47 mM; required
Bacillus megaterium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
42000
-
8 * 42000, SDS-PAGE
Bacillus megaterium
4.2.1.11
335000
-
gel filtration
Bacillus megaterium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Bacillus megaterium
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
spore
germinated
Bacillus megaterium
-
4.2.1.11
vegetative
-
Bacillus megaterium
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
-
Bacillus megaterium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
in the frozen state, stable for 6 months
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33609
Bacillus megaterium
phosphoenolpyruvate
-
33609
Bacillus megaterium
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
8 * 42000, SDS-PAGE
Bacillus megaterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8
-
-
Bacillus megaterium