BRENDA - Enzyme Database

Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties

Rider, C.C.; Taylor, C.B.; Biochim. Biophys. Acta 365, 285-300 (1974)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.12
-
2-phosphoglycerate
muscle enzyme
Rattus norvegicus
4.2.1.11
0.14
-
2-phosphoglycerate
liver enzyme
Rattus norvegicus
4.2.1.11
0.31
-
phosphoenolpyruvate
liver enzyme
Rattus norvegicus
4.2.1.11
0.37
-
phosphoenolpyruvate
muscle enzyme
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
2 * 49000, muscle enzyme, SDS-PAGE
Rattus norvegicus
4.2.1.11
53000
-
2 * 53000, liver enzyme, SDS-PAGE
Rattus norvegicus
4.2.1.11
91000
-
liver enzyme, gel filtration
Rattus norvegicus
4.2.1.11
93000
-
muscle enzyme, gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
liver enzyme; muscle enzyme
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
adipose tissue
enolase I
Rattus norvegicus
-
4.2.1.11
blood
enolase I
Rattus norvegicus
-
4.2.1.11
heart
enolase I and III
Rattus norvegicus
-
4.2.1.11
kidney
enolase I
Rattus norvegicus
-
4.2.1.11
liver
enolase I
Rattus norvegicus
-
4.2.1.11
muscle
contains only enolase III
Rattus norvegicus
-
4.2.1.11
spleen
enolase I
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
87
-
muscle enolase
Rattus norvegicus
4.2.1.11
120
-
muscle enolase
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
4°C, stable for several months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33604
Rattus norvegicus
phosphoenolpyruvate
-
33604
Rattus norvegicus
r
4.2.1.11
phosphoenolpyruvate
-
33604
Rattus norvegicus
2-phospho-D-glycerate
-
33604
Rattus norvegicus
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 49000, muscle enzyme, SDS-PAGE; 2 * 53000, liver enzyme, SDS-PAGE
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.12
-
2-phosphoglycerate
muscle enzyme
Rattus norvegicus
4.2.1.11
0.14
-
2-phosphoglycerate
liver enzyme
Rattus norvegicus
4.2.1.11
0.31
-
phosphoenolpyruvate
liver enzyme
Rattus norvegicus
4.2.1.11
0.37
-
phosphoenolpyruvate
muscle enzyme
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
2 * 49000, muscle enzyme, SDS-PAGE
Rattus norvegicus
4.2.1.11
53000
-
2 * 53000, liver enzyme, SDS-PAGE
Rattus norvegicus
4.2.1.11
91000
-
liver enzyme, gel filtration
Rattus norvegicus
4.2.1.11
93000
-
muscle enzyme, gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
liver enzyme; muscle enzyme
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
adipose tissue
enolase I
Rattus norvegicus
-
4.2.1.11
blood
enolase I
Rattus norvegicus
-
4.2.1.11
heart
enolase I and III
Rattus norvegicus
-
4.2.1.11
kidney
enolase I
Rattus norvegicus
-
4.2.1.11
liver
enolase I
Rattus norvegicus
-
4.2.1.11
muscle
contains only enolase III
Rattus norvegicus
-
4.2.1.11
spleen
enolase I
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
87
-
muscle enolase
Rattus norvegicus
4.2.1.11
120
-
muscle enolase
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
4°C, stable for several months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33604
Rattus norvegicus
phosphoenolpyruvate
-
33604
Rattus norvegicus
r
4.2.1.11
phosphoenolpyruvate
-
33604
Rattus norvegicus
2-phospho-D-glycerate
-
33604
Rattus norvegicus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 49000, muscle enzyme, SDS-PAGE; 2 * 53000, liver enzyme, SDS-PAGE
Rattus norvegicus