BRENDA - Enzyme Database

Significance of the enzymatic properties of yeast S39A enolase to the catalytic mechanism

Brewer, J.M.; Glover, C.V.C.; Holland, M.J.; Lebioda, L.; Biochim. Biophys. Acta 1383, 351-355 (1998)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
S39A
mutant of isoenzyme 1, relative maximal velocity of 0.01% and an activation constant for Mg2+ ca. 10fold higher, compared with the native enzyme
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
phosphonoacetohydroxamate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.032
-
2-phosphoglycerate
in presence of 1 mM Mg2+, native enzyme
Saccharomyces cerevisiae
4.2.1.11
0.131
-
2-phosphoglycerate
in presence of 1 mM Mg2+, mutant enzyme S39A
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
-
mutant S39A of isoenzyme 1
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33603
Saccharomyces cerevisiae
phosphoenolpyruvate
-
33603
Saccharomyces cerevisiae
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.018
0.019
2-phosphoglycerate
mutant enzyme S39A, pH 7.8, 21-23°C
Saccharomyces cerevisiae
4.2.1.11
230
-
2-phosphoglycerate
native enolase, pH 7.8, 21-23°C
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
S39A
mutant of isoenzyme 1, relative maximal velocity of 0.01% and an activation constant for Mg2+ ca. 10fold higher, compared with the native enzyme
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
phosphonoacetohydroxamate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.032
-
2-phosphoglycerate
in presence of 1 mM Mg2+, native enzyme
Saccharomyces cerevisiae
4.2.1.11
0.131
-
2-phosphoglycerate
in presence of 1 mM Mg2+, mutant enzyme S39A
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33603
Saccharomyces cerevisiae
phosphoenolpyruvate
-
33603
Saccharomyces cerevisiae
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.018
0.019
2-phosphoglycerate
mutant enzyme S39A, pH 7.8, 21-23°C
Saccharomyces cerevisiae
4.2.1.11
230
-
2-phosphoglycerate
native enolase, pH 7.8, 21-23°C
Saccharomyces cerevisiae