BRENDA - Enzyme Database

Purification and properties of enolase from swine kidney

Oh, S.K.; Brewer, J.M.; Arch. Biochem. Biophys. 157, 491-499 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.2.1.11
Mg2+, 20% sucrose or glycerol stabilize enzyme in dilute solutions
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
substrate inhibition
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
-
Sus scrofa
4.2.1.11
0.05
-
2-phosphoglycerate
-
Sus scrofa
4.2.1.11
0.11
-
phosphoenolpyruvate
-
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
KCl
required for maximal activity
Sus scrofa
4.2.1.11
Mg2+
Km: 0.43 mM
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
52000
-
2 * 52000, SDS-PAGE
Sus scrofa
4.2.1.11
88000
-
sedimentation velocity measurement
Sus scrofa
4.2.1.11
110000
-
gel filtration
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
kidney
-
Sus scrofa
-
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
4C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33602
Sus scrofa
phosphoenolpyruvate
-
33602
Sus scrofa
r
4.2.1.11
phosphoenolpyruvate
-
33602
Sus scrofa
2-phospho-D-glycerate
-
33602
Sus scrofa
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 52000, SDS-PAGE
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
4.2.1.11
Mg2+, 20% sucrose or glycerol stabilize enzyme in dilute solutions
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
substrate inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
-
Sus scrofa
4.2.1.11
0.05
-
2-phosphoglycerate
-
Sus scrofa
4.2.1.11
0.11
-
phosphoenolpyruvate
-
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
KCl
required for maximal activity
Sus scrofa
4.2.1.11
Mg2+
Km: 0.43 mM
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
52000
-
2 * 52000, SDS-PAGE
Sus scrofa
4.2.1.11
88000
-
sedimentation velocity measurement
Sus scrofa
4.2.1.11
110000
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
kidney
-
Sus scrofa
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
4C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33602
Sus scrofa
phosphoenolpyruvate
-
33602
Sus scrofa
r
4.2.1.11
phosphoenolpyruvate
-
33602
Sus scrofa
2-phospho-D-glycerate
-
33602
Sus scrofa
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 52000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Sus scrofa