BRENDA - Enzyme Database

Isolation and characterization of enolase from the rhesus monkey (Macaca mulatta)

Winstead, J.A.; Biochemistry 11, 1046-1051 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Ca2+
-
Macaca mulatta
4.2.1.11
phosphate
-
Macaca mulatta
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
-
Macaca mulatta
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Co2+
absolute requirement for divalent metal ions, 9% of the activity with Mg2+
Macaca mulatta
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective
Macaca mulatta
4.2.1.11
Mn2+
46% of the activation with Mg2+; absolute requirement for divalent metal ions
Macaca mulatta
4.2.1.11
Zn2+
absolute requirement for divalent metal ions, 35% of the activation with Mg2+
Macaca mulatta
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
82000
-
low speed sedimentation without reaching equilibrium
Macaca mulatta
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Macaca mulatta
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Macaca mulatta
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
skeletal muscle
-
Macaca mulatta
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
86.54
-
-
Macaca mulatta
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.11
4°C, 70% saturated ammonium sulfate solution, stable for several months
Macaca mulatta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33601
Macaca mulatta
phosphoenolpyruvate
-
33601
Macaca mulatta
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
-
Macaca mulatta
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
9
pH 6.0: about 35% of maximal activity, pH 9.0: about 30% of maximal activity
Macaca mulatta
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Ca2+
-
Macaca mulatta
4.2.1.11
phosphate
-
Macaca mulatta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
additional information
-
additional information
-
Macaca mulatta
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Co2+
absolute requirement for divalent metal ions, 9% of the activity with Mg2+
Macaca mulatta
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective
Macaca mulatta
4.2.1.11
Mn2+
46% of the activation with Mg2+; absolute requirement for divalent metal ions
Macaca mulatta
4.2.1.11
Zn2+
absolute requirement for divalent metal ions, 35% of the activation with Mg2+
Macaca mulatta
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
82000
-
low speed sedimentation without reaching equilibrium
Macaca mulatta
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Macaca mulatta
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
skeletal muscle
-
Macaca mulatta
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
86.54
-
-
Macaca mulatta
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.11
4°C, 70% saturated ammonium sulfate solution, stable for several months
Macaca mulatta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33601
Macaca mulatta
phosphoenolpyruvate
-
33601
Macaca mulatta
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
-
Macaca mulatta
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
9
pH 6.0: about 35% of maximal activity, pH 9.0: about 30% of maximal activity
Macaca mulatta