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Literature summary extracted from

  • Kisiel, W.; Graf, G.
    Purification and characterization of carbonic anhydrase from Pisum sativum (1972), Phytochemistry, 11, 113-117.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide
-
 Pisum sativum
4.2.1.1 HCO3- substrate inhibition above 0.1 M Pisum sativum
4.2.1.1 Hg2+
-
 Pisum sativum
4.2.1.1 o-Iodosobenzoate
-
 Pisum sativum
4.2.1.1 PCMB 0.25 mM, complete inhibition after 10 min Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 30
-
 HCO3-
-
 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zinc contains 6 mol of zinc per mol of enzyme Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 30000
-
 6 * 30000, SDS-PAGE Pisum sativum
4.2.1.1 194000
-
 gel filtration, meniscus depletion equilibrium sedimentation, analytical ultracentrifugation Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Pisum sativum H2CO3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 hexamer 6 * 30000, SDS-PAGE Pisum sativum