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Literature summary extracted from

  • Sundaraju, B.; Antson, A.A.; Phillips, R.S.; Demidkina, T.V.; Barbolina, M.V.; Gollnick, P.; Dodson, G.G.; Wilson, K.S.
    The crystal structure of Citrobacter freundii tyrosine phenol-lyase complexed with 3-(4 -hydroxyphenyl)propionic acid, together with site-directed mutagenesis and kinetic analysis, demonstrates that arginine 381 is required for substrate specificity (1997), Biochemistry, 36, 6502-6510.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.99.2 tyrosine phenol-lyase complexed with 3-(4'-hydroxyphenyl)propionic acid Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.2 R381A beta-elimination activity has been reduced by 0.0001fold compared to wild type enzyme Citrobacter freundii
4.1.99.2 R381I no detectable beta-elimination activity with L-Tyr as substrate Citrobacter freundii
4.1.99.2 R381V no detectable beta-elimination activity with L-Tyr as substrate Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter freundii P31013 wild type enzyme and mutant enzymes R381A, R381V and R381I
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 O-benzoyl-L-Ser + H2O
-
 Citrobacter freundii ?
-
 ?
4.1.99.2 O-benzyl-L-Cys + H2O
-
 Citrobacter freundii ?
-
 ?
4.1.99.2 O-benzyl-L-Ser + H2O
-
 Citrobacter freundii ?
-
 ?
4.1.99.2 S-benzyl-L-Cys + H2O
-
 Citrobacter freundii ? + pyruvate + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.2 additional information
-
 additional information the turnover-numbers of the mutant enzyme R381I, R381V and R381A for the substrates S-(o-nitrophenyl)-L-Cys, 3-chloro-L-Ala, O-benzoyl-L-Ser and S-methyl-L-Cys are comparable to those of the wild-type enzyme Citrobacter freundii
4.1.99.2 0.5
-
 S-benzyl-L-Cys wild-type enzyme Citrobacter freundii
4.1.99.2 0.9
-
 S-methyl-L-Cys wild-type enzyme Citrobacter freundii
4.1.99.2 1.7
-
 S-ethyl-L-Cys wild-type enzyme Citrobacter freundii
4.1.99.2 2.2
-
 L-Tyr wild-type enzyme Citrobacter freundii
4.1.99.2 3
-
 3-chloro-L-Ala wild-type enzyme Citrobacter freundii
4.1.99.2 3.9
-
 O-benzyl-L-Cys wild-type enzyme Citrobacter freundii
4.1.99.2 5.1
-
 S-(o-nitrophenyl)-L-Cys wild-type enzyme Citrobacter freundii
4.1.99.2 8.3
-
 O-benzoyl-L-Ser wild-type enzyme Citrobacter freundii
4.1.99.2 9.7
-
 S-(o-nitrophenyl)-L-Cys wild-type enzyme Citrobacter freundii